Key features and details
- Rabbit polyclonal to KAT5 / Tip60
- Suitable for: ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-KAT5 / Tip60 antibody
See all KAT5 / Tip60 primary antibodies
DescriptionRabbit polyclonal to KAT5 / Tip60
Specificityab23886 gives nuclear staining for Tip60 in interphase cells (see below), which is lost upon entry into mitosis (not shown). This signal can be successfully quenched using the immunizing peptide ab26349.
Tested applicationsSuitable for: ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Drosophila melanogaster, Zebrafish
- This antibody gave a positive signal in MCF7 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab23886 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
FunctionCatalytic subunit of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome-DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Directly acetylates and activates ATM. In case of HIV-1 infection, interaction with the viral Tat protein leads to KAT5 polyubiquitination and targets it to degradation.
Sequence similaritiesBelongs to the MYST (SAS/MOZ) family.
Contains 1 C2HC-type zinc finger.
modificationsSumoylated by UBE2I at Lys-430 and Lys-451, leading to increase of its histone acetyltransferase activity in UV-induced DNA damage response, as well as its translocation to nuclear bodies.
Phosphorylated on Ser-86 and Ser-90; enhanced during G2/M phase. Phosphorylated form has a higher activity.
Ubiquitinated by MDM2, leading to its proteasome-dependent degradation.
Cellular localizationNucleus > nucleolus. Cytoplasm > perinuclear region. Upon stimulation with EDN1, it is exported from the nucleus to the perinuclear region and UV irradiation induces translocation into punctuate subnuclear structures named nuclear bodies.
- Information by UniProt
- 60 kDa Tat interactive protein antibody
- 60 kDa Tat-interactive protein antibody
- cPLA(2) interacting protein antibody
ab23886 staining KAT5 / Tip60 in human primary fibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.2% Triton X-100 and blocked with 2% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/500 in PBS + 2% BSA) for 2 hours. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.
HeLa cells were plated on poly-D lysine coated cover slips, fixed with 4% w/v paraformaldehyde (10 min), permeabilised in 0.1% Triton X-100 in PBS (10 min), incubated for 1 hour at 37°C with ab23886 and stained with a FITC-conjugated secondary antibody and DAPI. Left panel: FITC signal. Right panel: FITC+DAPI.
ab23886 gives a specific nuclear staining for Tip60. Nucleoli are stained more intensely, as also demonstrated by co-staining with known nucleolar makers (not shown). The signal is blocked by pre-incubation of ab23886 with the cognate immunogenic peptide.
ICC/IF image of ab23886 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab23886, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab23886 has been referenced in 16 publications.
- Panikker P et al. Restoring Tip60 HAT/HDAC2 Balance in the Neurodegenerative Brain Relieves Epigenetic Transcriptional Repression and Reinstates Cognition. J Neurosci 38:4569-4583 (2018). PubMed: 29654189
- Lee HS et al. The chromatin remodeler RSF1 controls centromeric histone modifications to coordinate chromosome segregation. Nat Commun 9:3848 (2018). PubMed: 30242288
- Zhang Y et al. Histone H3K27 methylation modulates the dynamics of FANCD2 on chromatin to facilitate NHEJ and genome stability. J Cell Sci 131:N/A (2018). PubMed: 29760279
- Baarlink C et al. A transient pool of nuclear F-actin at mitotic exit controls chromatin organization. Nat Cell Biol 19:1389-1399 (2017). PubMed: 29131140
- Falcone Ferreyra ML et al. AtPDCD5 Plays a Role in Programmed Cell Death after UV-B Exposure in Arabidopsis. Plant Physiol 170:2444-60 (2016). PubMed: 26884483