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Host species: Goat
Conjugation: Horse Radish Peroxidase
Not sure if the extra band below what appears to be the correct size for
Drosophila Tip60 (~ 61 KDa) is an artifact, breakdown or modified form.
I had to let the detection die for about 1.5 hrs before exposing to film.
Next time I will dilute the Tip60 Ab to 1:1,500 or higher depending on sample concentration. Also, another blot blocked with BSA was awful!
Abcam user community
Submitted Nov 22 2011