Overview

  • Product name

    Anti-KAT7 / Hbo1 / MYST2 antibody [EPR18473]
    See all KAT7 / Hbo1 / MYST2 primary antibodies
  • Description

    Rabbit monoclonal [EPR18473] to KAT7 / Hbo1 / MYST2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human KAT7/ Hbo1/ MYST2 aa 1-200. The exact sequence is proprietary.
    Database link: O95251

  • Positive control

    • WB: HeLa, MCF7, NIH/3T3, Jurkat, C6 and RAW 264.7 cell lysates; human breast cancer lysate. IHC-P: human colon, human testis, mouse colon, rat colon and rat testis tissues. ICC/IF: HEK-293 cells. IP: Jurkat whole cell lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab190908 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 75 kDa (predicted molecular weight: 71 kDa).
IHC-P 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/2000.
IP 1/60.

Target

  • Function

    Component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. Through chromatin acetylation it may regulate DNA replication and act as a coactivator of TP53-dependent transcription. Specifically represses AR-mediated transcription.
  • Tissue specificity

    Ubiquitously expressed, with highest levels in testis.
  • Sequence similarities

    Belongs to the MYST (SAS/MOZ) family.
    Contains 1 C2HC-type zinc finger.
  • Domain

    The C2HC-type zinc finger is required for interaction with MCM2 and ORC1L.
    The N-terminus is involved in transcriptional repression, while the C-terminus mediates AR-interaction.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus > nucleoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Hbo 1 antibody
    • HBO1 antibody
    • HBOa antibody
    • Histone acetyltransferase binding to hORC1 antibody
    • Histone acetyltransferase binding to ORC1 antibody
    • Histone acetyltransferase KAT7 antibody
    • Histone acetyltransferase MYST2 antibody
    • K(lysine) acetyltransferase 7 antibody
    • KAT 7 antibody
    • KAT7 antibody
    • Lysine acetyltransferase 7 antibody
    • MOZ antibody
    • MOZ YBF2/SAS3 SAS2 and TIP60 protein antibody
    • MOZ, YBF2/SAS3, SAS2 and TIP60 protein 2 antibody
    • MYST 2 antibody
    • MYST histone acetyltransferase 2 antibody
    • MYST protein 2 antibody
    • MYST-2 antibody
    • MYST2 antibody
    • MYST2_HUMAN antibody
    • SAS 2 antibody
    • SAS2 and TIP60 protein 2 antibody
    • TIP60 protein 2 antibody
    • YBF2/SAS3 antibody
    • ZC2HC7 antibody
    see all

Images

  • All lanes : Anti-KAT7 / Hbo1 / MYST2 antibody [EPR18473] (ab190908) at 1/5000 dilution

    Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate
    Lane 2 : MCF7 (Human breast adenocarcinoma cell line) cell lysate
    Lane 3 : NIH/3T3 (Mouse embryonic fibroblast cell line) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 71 kDa
    Observed band size: 75 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-KAT7 / Hbo1 / MYST2 antibody [EPR18473] (ab190908) at 1/5000 dilution

    Lane 1 : C6 (Rat glial tumor cells) cell lysate
    Lane 2 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 71 kDa
    Observed band size: 75 kDa why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-KAT7 / Hbo1 / MYST2 antibody [EPR18473] (ab190908) at 1/1000 dilution + human breast cancer lysate at 40 µg

    Secondary
    Goat Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/50000 dilution

    Predicted band size: 71 kDa
    Observed band size: 75 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemical analysis of paraffin-embedded human testis tissue labeling KAT7 / Hbo1 / MYST2 with ab190908 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on human testis is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling KAT7 / Hbo1 / MYST2 with ab190908 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on mouse colon is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling KAT7 / Hbo1 / MYST2 with ab190908 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on rat testis is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293 (Human epithelial cells from embryonic kidney) cells labeling KAT7 / Hbo1 / MYST2 with ab190908 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing nuclear staining on HEK-293 cell line. The nuclear counter stain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/500 dilution (red).
    The negative controls are as follows:
    -ve control 1: ab190908 at 1/2000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary at 1/500 dilution.
    -ve control 2: Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary at 1/500 dilution.

  • KAT7 / Hbo1 / MYST2 was immunoprecipitated from 1mg of Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate with ab190908 at 1/60 dilution. Western blot was performed from the immunoprecipitate using ab190908 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Jurkat whole cell lysate 10ug (Input). 

    Lane 2: ab190908 IP in Jurkat whole cell lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab190908 in Jurkat whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 10 seconds.

References

ab190908 has not yet been referenced specifically in any publications.

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