Overview

  • Product name

    Anti-KAT7 / Hbo1 / MYST2 antibody [EPR7194(B)]
    See all KAT7 / Hbo1 / MYST2 primary antibodies
  • Description

    Rabbit monoclonal [EPR7194(B)] to KAT7 / Hbo1 / MYST2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human KAT7/ Hbo1/ MYST2 aa 50-150. The exact sequence is proprietary.
    Database link: O95251

  • Positive control

    • HeLa, Jurkat, MCF7, and HepG2 whole cell lysate (ab7900); Permeabilized HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab124993 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 71 kDa (predicted molecular weight: 71 kDa).
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    • Function

      Component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. Through chromatin acetylation it may regulate DNA replication and act as a coactivator of TP53-dependent transcription. Specifically represses AR-mediated transcription.
    • Tissue specificity

      Ubiquitously expressed, with highest levels in testis.
    • Sequence similarities

      Belongs to the MYST (SAS/MOZ) family.
      Contains 1 C2HC-type zinc finger.
    • Domain

      The C2HC-type zinc finger is required for interaction with MCM2 and ORC1L.
      The N-terminus is involved in transcriptional repression, while the C-terminus mediates AR-interaction.
    • Post-translational
      modifications

      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization

      Nucleus > nucleoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • Hbo 1 antibody
      • HBO1 antibody
      • HBOa antibody
      • Histone acetyltransferase binding to hORC1 antibody
      • Histone acetyltransferase binding to ORC1 antibody
      • Histone acetyltransferase KAT7 antibody
      • Histone acetyltransferase MYST2 antibody
      • K(lysine) acetyltransferase 7 antibody
      • KAT 7 antibody
      • KAT7 antibody
      • Lysine acetyltransferase 7 antibody
      • MOZ antibody
      • MOZ YBF2/SAS3 SAS2 and TIP60 protein antibody
      • MOZ, YBF2/SAS3, SAS2 and TIP60 protein 2 antibody
      • MYST 2 antibody
      • MYST histone acetyltransferase 2 antibody
      • MYST protein 2 antibody
      • MYST-2 antibody
      • MYST2 antibody
      • MYST2_HUMAN antibody
      • SAS 2 antibody
      • SAS2 and TIP60 protein 2 antibody
      • TIP60 protein 2 antibody
      • YBF2/SAS3 antibody
      • ZC2HC7 antibody
      see all

    Images

    • All lanes : Anti-KAT7 / Hbo1 / MYST2 antibody [EPR7194(B)] (ab124993) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : MCF7 cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 71 kDa

    • ab124993 at 1/100 dilution staining KAT7 / Hbo1 / MYST2 in permeabilized HeLa cells by Flow cytometry (red) or negative control (green).
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Miotto B  et al. The RBBP6/ZBTB38/MCM10 axis regulates DNA replication and common fragile site stability. Cell Rep 7:575-87 (2014). WB . Read more (PubMed: 24726359) »
    See 1 Publication for this product

    Customer reviews and Q&As

    Answer

    Thank you for your phone call.
    The overall homology between Human and Xenopus is 93%. Thus, in general there is good chance for an antibody raised against human to work in Xenopus.
    For ab31506 the homology of the immunogen with Xenopus is 100%.
    For ab87100 the homology of the immunogen with Xenopus is 90%. For the other Hbo1 antibodies I currently do not have information about the immunogen range. I can check with the lab if you like - please let me know.
    None of these antibodies has been tested in IP.
    DISCOUNT CODE: xxx
    Expiration date: xxx
    I am very pleased to hear you would like to accept our offer and test ab31506 in Xenopus and IP. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for Xenopus/IP and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.
    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.
    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.
    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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