RecombinantRabMAb

Recombinant Anti-KAT7 / Hbo1 / MYST2 antibody [EPR7194(B)] - BSA and Azide free (ab248087)

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Western blot - Anti-KAT7 / Hbo1 / MYST2 antibody [EPR7194(B)] - BSA and Azide free (ab248087)
  • Flow Cytometry - Anti-KAT7 / Hbo1 / MYST2 antibody [EPR7194(B)] - BSA and Azide free (ab248087)
  • OI-RD Scanning - Anti-KAT7 / Hbo1 / MYST2 antibody [EPR7194(B)] - BSA and Azide free (ab248087)
  • Anti-KAT7 / Hbo1 / MYST2 antibody [EPR7194(B)] - BSA and Azide free (ab248087)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7194(B)] to KAT7 / Hbo1 / MYST2 - BSA and Azide free
  • Suitable for: WB, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-KAT7 / Hbo1 / MYST2 antibody [EPR7194(B)] - BSA and Azide free
    See all KAT7 / Hbo1 / MYST2 primary antibodies

  • Description

    Rabbit monoclonal [EPR7194(B)] to KAT7 / Hbo1 / MYST2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,ICC/IF,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human KAT7/ Hbo1/ MYST2 aa 50-150. The exact sequence is proprietary.
    Database link: O95251

  • General notes

    Ab248087 is the carrier-free version of ab124993. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab248087 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab248087 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 71 kDa (predicted molecular weight: 71 kDa).
Flow Cyt Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for ICC,ICC/IF,IHC-P or IP.

    • Target

    • Function

      Component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. Through chromatin acetylation it may regulate DNA replication and act as a coactivator of TP53-dependent transcription. Specifically represses AR-mediated transcription.

    • Tissue specificity

      Ubiquitously expressed, with highest levels in testis.

    • Sequence similarities

      Belongs to the MYST (SAS/MOZ) family.
      Contains 1 C2HC-type zinc finger.

    • Domain

      The C2HC-type zinc finger is required for interaction with MCM2 and ORC1L.
      The N-terminus is involved in transcriptional repression, while the C-terminus mediates AR-interaction.

    • Post-translational
      modifications

      Phosphorylated upon DNA damage, probably by ATM or ATR.

    • Cellular localization

      Nucleus > nucleoplasm.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Hbo 1 antibody
      • HBO1 antibody
      • HBOa antibody
      • Histone acetyltransferase binding to hORC1 antibody
      • Histone acetyltransferase binding to ORC1 antibody
      • Histone acetyltransferase KAT7 antibody
      • Histone acetyltransferase MYST2 antibody
      • K(lysine) acetyltransferase 7 antibody
      • KAT 7 antibody
      • KAT7 antibody
      • Lysine acetyltransferase 7 antibody
      • MOZ antibody
      • MOZ YBF2/SAS3 SAS2 and TIP60 protein antibody
      • MOZ, YBF2/SAS3, SAS2 and TIP60 protein 2 antibody
      • MYST 2 antibody
      • MYST histone acetyltransferase 2 antibody
      • MYST protein 2 antibody
      • MYST-2 antibody
      • MYST2 antibody
      • MYST2_HUMAN antibody
      • SAS 2 antibody
      • SAS2 and TIP60 protein 2 antibody
      • TIP60 protein 2 antibody
      • YBF2/SAS3 antibody
      • ZC2HC7 antibody
      see all

    Images

    • Western blot - Anti-KAT7 / Hbo1 / MYST2 antibody [EPR7194(B)] - BSA and Azide free (ab248087)
      Western blot - Anti-KAT7 / Hbo1 / MYST2 antibody [EPR7194(B)] - BSA and Azide free (ab248087)
      All lanes : Anti-KAT7 / Hbo1 / MYST2 antibody [EPR7194(B)] (ab124993) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : MCF7 cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 71 kDa



      This data was developed using ab124993, the same antibody clone in a different buffer formulation.

    • Flow Cytometry - Anti-KAT7 / Hbo1 / MYST2 antibody [EPR7194(B)] - BSA and Azide free (ab248087)
      Flow Cytometry - Anti-KAT7 / Hbo1 / MYST2 antibody [EPR7194(B)] - BSA and Azide free (ab248087)
      This data was developed using ab124993, the same antibody clone in a different buffer formulation.ab124993 at 1/100 dilution staining KAT7 / Hbo1 / MYST2 in permeabilized HeLa cells by Flow cytometry (red) or negative control (green).
    • OI-RD Scanning - Anti-KAT7 / Hbo1 / MYST2 antibody [EPR7194(B)] - BSA and Azide free (ab248087)
      OI-RD Scanning - Anti-KAT7 / Hbo1 / MYST2 antibody [EPR7194(B)] - BSA and Azide free (ab248087)
      This data was developed using ab124993, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-KAT7 / Hbo1 / MYST2 antibody [EPR7194(B)] - BSA and Azide free (ab248087)
      Anti-KAT7 / Hbo1 / MYST2 antibody [EPR7194(B)] - BSA and Azide free (ab248087)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab248087? Please let us know so that we can cite the reference in this datasheet.

    ab248087 has not yet been referenced specifically in any publications.

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