Product nameAnti-KAT8 / MYST1 / MOF antibody
See all KAT8 / MYST1 / MOF primary antibodies
DescriptionRabbit polyclonal to KAT8 / MYST1 / MOF
Tested applicationsSuitable for: IHC-P, WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Cow, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat, Elephant
Synthetic peptide corresponding to a region between residue 1 and 50 of human KAT8/ MYST1/ MOF (BAB13924.1).
- Whole cell lysate from HeLa cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab72056 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
|WB||1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 52 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionHistone acetyltransferase which may be involved in transcriptional activation. May influence the function of ATM.
Sequence similaritiesBelongs to the MYST (SAS/MOZ) family.
Contains 1 C2HC-type zinc finger.
Contains 1 chromo domain.
- Information by UniProt
- Histone acetyltransferase KAT8 antibody
- Histone acetyltransferase MYST1 antibody
- hMOF antibody
All lanes : Anti-KAT8 / MYST1 / MOF antibody (ab72056) at 0.4 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Predicted band size: 52 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa, 19 kDa, 28 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling KAT8 / MYST1 / MOF with ab72056 at 1/1000 (1µg/ml). Detection: DAB.
Detection of Human KAT8 / MYST1 / MOF by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab72056 at 3 µg/mg lysate. Subsequent WB detection was done using ab72056 at 1 µg/ml.
IHC image of ab72056 staining in human normal cervix formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab72056, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
This product has been referenced in:
- Li L & Wang Y Cross-talk between the H3K36me3 and H4K16ac histone epigenetic marks in DNA double-strand break repair. J Biol Chem 292:11951-11959 (2017). Read more (PubMed: 28546430) »
- Liu Y et al. MDM2-MOF-H4K16ac axis contributes to tumorigenesis induced by Notch. FEBS J 281:3315-24 (2014). Read more (PubMed: 24898892) »