Overview

  • Product name

  • Description

    Rabbit polyclonal to KAT9 / Elp3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human KAT9/ Elp3 aa 1-85.
    Database link: Q9H9T3

  • Positive control

    • WB: Jurkat, K562 and HEK-293 whole cell lysates. IHC-P: Human kidney tissue. ICC/IF: HeLa cells. IP: HEK-293 whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab236800 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/5000. Predicted molecular weight: 62 kDa.
ICC/IF 1/50 - 1/200.
IP 1/200 - 1/2000.
IHC-P 1/200 - 1/500.

Target

  • Function

    Catalytic histone acetyltransferase subunit of the RNA polymerase II elongator complex, which is a component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation. Elongator may play a role in chromatin remodeling and is involved in acetylation of histones H3 and probably H4. May also have a methyltransferase activity.
  • Sequence similarities

    Belongs to the ELP3 family.
    Contains 1 N-acetyltransferase domain.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • DKFZp761L098 antibody
    • Elongation protein 3 homolog (S. cerevisiae) antibody
    • Elongation protein 3 homolog antibody
    • ELONGATION PROTEIN 3, S. CEREVISIAE, HOMOLOG OF antibody
    • elongator acetyltransferase complex subunit 3 antibody
    • Elongator complex protein 3 antibody
    • elp3 antibody
    • ELP3_HUMAN antibody
    • FLJ10422 antibody
    • hELP3 antibody
    • Hypothetical protein DKFZp761L098 antibody
    • Kat9 antibody
    see all

Images

  • All lanes : Anti-KAT9 / Elp3 antibody (ab236800) at 1/500 dilution

    Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 2 : K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate
    Lane 3 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 62 kDa

  • Paraffin-embedded human kidney tissue stained for KAT9 / Elp3 using ab236800 at 1/400 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • 4% formaldehyde-fixed, 0.2% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for KAT9 / Elp3 (green) using ab236800 at 1/133 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L). Counter-stained with DAPI.

  • KAT9 / Elp3 was immunoprecipitated from 500 µg HEK-293 (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with ab236800 at 1/200 dilution. 

    Lane 1: Rabbit control IgG IP in HEK-293 whole cell lysate.

    Lane 2: ab236800 IP in HEK-293 whole cell lysate.

    Lane 3: HEK-293 whole cell lysate 20 µg (Input).

    For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)

References

ab236800 has not yet been referenced specifically in any publications.

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