Key features and details
- Rabbit polyclonal to KAT9 / Elp3
- Suitable for: WB, ICC/IF, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-KAT9 / Elp3 antibody
See all KAT9 / Elp3 primary antibodies
DescriptionRabbit polyclonal to KAT9 / Elp3
Tested applicationsSuitable for: WB, ICC/IF, IP, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human KAT9/ Elp3 aa 1-85.
Database link: Q9H9T3
- WB: Jurkat, K562 and HEK-293 whole cell lysates. IHC-P: Human kidney tissue. ICC/IF: HeLa cells. IP: HEK-293 whole cell lysate.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%
Our Abpromise guarantee covers the use of ab236800 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000. Predicted molecular weight: 62 kDa.|
|ICC/IF||1/50 - 1/200.|
|IP||1/200 - 1/2000.|
|IHC-P||1/200 - 1/500.|
FunctionCatalytic histone acetyltransferase subunit of the RNA polymerase II elongator complex, which is a component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation. Elongator may play a role in chromatin remodeling and is involved in acetylation of histones H3 and probably H4. May also have a methyltransferase activity.
Sequence similaritiesBelongs to the ELP3 family.
Contains 1 N-acetyltransferase domain.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- DKFZp761L098 antibody
- Elongation protein 3 homolog (S. cerevisiae) antibody
- Elongation protein 3 homolog antibody
All lanes : Anti-KAT9 / Elp3 antibody (ab236800) at 1/500 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate
Lane 3 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 62 kDa
Paraffin-embedded human kidney tissue stained for KAT9 / Elp3 using ab236800 at 1/400 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
4% formaldehyde-fixed, 0.2% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for KAT9 / Elp3 (green) using ab236800 at 1/133 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L). Counter-stained with DAPI.
KAT9 / Elp3 was immunoprecipitated from 500 µg HEK-293 (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with ab236800 at 1/200 dilution.
Lane 1: Rabbit control IgG IP in HEK-293 whole cell lysate.
Lane 2: ab236800 IP in HEK-293 whole cell lysate.
Lane 3: HEK-293 whole cell lysate 20 µg (Input).
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236800 has not yet been referenced specifically in any publications.