Overview

  • Product name

    Anti-KAT9 / Elp3 antibody [EPR18472]
    See all KAT9 / Elp3 primary antibodies
  • Description

    Rabbit monoclonal [EPR18472] to KAT9 / Elp3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human KAT9/ Elp3 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9H9T3

  • Positive control

    • WB: HeLa, Jurkat, 293, HepG2, MCF7, C6, RAW264.7, PC-12 and NIH/3T3 whole cell lysates; Human fetal brain, fetal heart and fetal kidney lysates; Mouse and rat brain, heart, kidney and spleen lysates. ICC/IF: HeLa cells. IP: MCF7 whole cell lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab190907 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/70.
ICC/IF 1/2000.
WB 1/2000. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).

Target

  • Function

    Catalytic histone acetyltransferase subunit of the RNA polymerase II elongator complex, which is a component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation. Elongator may play a role in chromatin remodeling and is involved in acetylation of histones H3 and probably H4. May also have a methyltransferase activity.
  • Sequence similarities

    Belongs to the ELP3 family.
    Contains 1 N-acetyltransferase domain.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • DKFZp761L098 antibody
    • Elongation protein 3 homolog (S. cerevisiae) antibody
    • Elongation protein 3 homolog antibody
    • ELONGATION PROTEIN 3, S. CEREVISIAE, HOMOLOG OF antibody
    • elongator acetyltransferase complex subunit 3 antibody
    • Elongator complex protein 3 antibody
    • elp3 antibody
    • ELP3_HUMAN antibody
    • FLJ10422 antibody
    • hELP3 antibody
    • Hypothetical protein DKFZp761L098 antibody
    • Kat9 antibody
    see all

Images

  • All lanes : Anti-KAT9 / Elp3 antibody [EPR18472] (ab190907) at 1/5000 dilution

    Lanes 1 & 7 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
    Lane 2 : K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysate
    Lane 3 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
    Lane 4 : 293 (Human epithelial cells from embryonic kidney) whole cell lysate
    Lane 5 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
    Lane 6 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution

    Predicted band size: 62 kDa
    Observed band size: 62 kDa


    Exposure time: 5 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-KAT9 / Elp3 antibody [EPR18472] (ab190907) at 1/2000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Human fetal heart lysate
    Lane 3 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 62 kDa
    Observed band size: 62 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-KAT9 / Elp3 antibody [EPR18472] (ab190907) at 1/2000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse heart lysate
    Lane 3 : Mouse kidney lysate
    Lane 4 : Mouse spleen lysate
    Lane 5 : Rat brain lysate
    Lane 6 : Rat heart lysate
    Lane 7 : Rat kidney lysate
    Lane 8 : Rat spleen lysate
    Lane 9 : C6 (Rat glial tumor cells) whole cell lysate
    Lane 10 : RAW264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 11 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
    Lane 12 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution

    Predicted band size: 62 kDa
    Observed band size: 62 kDa


    Exposure time: 5 seconds


    5% NFDM/TBST: Blocking and diluting buffer.

  • All lanes : Anti-KAT9 / Elp3 antibody [EPR18472] (ab190907) at 1/10000 dilution

    Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) cytosolic fraction lysate
    Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) nuclear fraction lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution

    Predicted band size: 62 kDa
    Observed band size: 62 kDa


    Exposure time: 15 seconds
  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling KAT9 / Elp3 with ab190907 at 1/2000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing cytoplasmic and nuclear staining on HeLa cells line.The staining pattern is in agreement with the data shown in the following publications: Cell 2009. 136, 551-64 (PMID: 19185337) and J Biol Chem. 2002. 277, 3047-3052 (PMID: 11714725). 

    The nuclear counterstain is DAPI (blue).

    Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

    The negative controls are as follows:-
    -ve control 1: ab190907 at 1/2000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

  • KAT9 / Elp3 was immunoprecipitated from 1mg of MCF7 (Human breast adenocarcinoma cell line) whole cell lysate with ab190907 at 1/70 dilution.

    Lane 1: MCF7 whole cell lysate 10ug (Input).

    Lane 2: ab190907 IP in MCF7 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab190907 in MCF7 whole cell lysate.

    Western blot was performed from the immunoprecipitate using ab190907 at 1/5000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds.

References

This product has been referenced in:

  • Maio N  et al. Dimeric ferrochelatase bridges ABCB7 and ABCB10 homodimers in an architecturally defined molecular complex required for heme biosynthesis. Haematologica N/A:N/A (2019). Read more (PubMed: 30765471) »
  • Kim KS  et al. Cytosolic HSC20 integrates de novo iron-sulfur cluster biogenesis with the CIAO1-mediated transfer to recipients. Hum Mol Genet 27:837-852 (2018). Read more (PubMed: 29309586) »
See all 2 Publications for this product

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