Anti-KATNAL2 antibody (ab184829)
Key features and details
- Rabbit polyclonal to KATNAL2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-KATNAL2 antibody
See all KATNAL2 primary antibodies -
Description
Rabbit polyclonal to KATNAL2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment corresponding to Human KATNAL2 aa 150-300.
Database link: Q8IYT4 -
Positive control
- IHC-P: Human thyroid gland, testis, fallopian tube, cerebral cortex and skeletal muscle tissue; WB: A-549 and Caco-2 whole cell lysate; ICC: U-2 OS whole cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab184829 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 61 kDa.
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IHC-P |
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100 |
Notes |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 61 kDa. |
IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100 |
Target
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Function
Severs microtubules in vitro in an ATP-dependent manner. This activity may promote rapid reorganization of cellular microtubule arrays. -
Sequence similarities
Belongs to the AAA ATPase family. Katanin p60 subunit A1 subfamily. A-like 2 sub-subfamily.
Contains 1 LisH domain. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 83473 Human
- Entrez Gene: 71206 Mouse
- Entrez Gene: 307253 Rat
- SwissProt: Q8IYT4 Human
- SwissProt: Q9D3R6 Mouse
- Unigene: 404137 Human
- Unigene: 35803 Mouse
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Alternative names
- DKFZP667C165 antibody
- Katanin p60 ATPase-containing subunit A-like 2 antibody
- Katanin p60 subunit A like 2 antibody
see all
Images
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Immunohistochemical analysis of human thyroid gland labeling KATNAL2 in the cytoplasm of glandular cells with ab184829 at a 1/20 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-KATNAL2 antibody (ab184829) at 0.4 µg/ml
Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 2 : Caco-2 (human colorectal adenocarcinoma cell line) whole cell lysate
Predicted band size: 61 kDaCorresponding KATNAL2 RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.
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Immunofluorescence analysis of hU-2 OS (human bone osteosarcoma epithelial cell line) whole cells labeling KATNAL2 in the nucleoplasm and intermediate filaments with ab184829 at 2 µg/ml.
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Immunohistochemical analysis of human testis tissue labeling KATNAL2 in the cytoplasm and membrane of seminiferous ducts with ab184829 at a 1/20 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of human fallopian tube tissue labeling KATNAL2 in the cytoplasm of glandular cells with ab184829 at a 1/20 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of human cerebral cortex tissue labeling KATNAL2 in the cytoplasm of neurons with ab184829 at a 1/20 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of human skeletal muscle tissue labeling KATNAL2 in the cytoplasm of myocytes with ab184829 at a 1/20 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab184829 has not yet been referenced specifically in any publications.