Key features and details
- Rabbit polyclonal to Kazrin
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Kazrin antibody
See all Kazrin primary antibodies
DescriptionRabbit polyclonal to Kazrin
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
Synthetic peptide corresponding to Human Kazrin aa 300-400 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in Kazrin over-expressing cell lines: HA tagged KazrinA and HA tagged KazrinE.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab74114 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 mg/ml. Detects a band of approximately 53,100 kDa (predicted molecular weight: 86 kDa).|
|ICC/IF||Use a concentration of 5 µg/ml.|
FunctionComponent of the cornified envelope of keratinocytes. May be involved in the interplay between adherens junctions and desmosomes. The function in the nucleus is not known.
Tissue specificityIsoform 2, isoform 3 and isoform 4 are expressed in several cell lines including keratinocytes and bladder and epidermoid carcinoma (at protein level). Isoform 2, isoform 3 and isoform 4 are expressed in hair follicle and interfollicular epidermis (at protein level).
Sequence similaritiesBelongs to the kazrin family.
Contains 3 SAM (sterile alpha motif) domains.
Cellular localizationCytoplasm > cytoskeleton and Cytoplasm. Cell junction > desmosome. Nucleus. Observed at the apical plasma mebrane of keratinocytes. Partially colocalizes with PPL and DP at desmosomes, and with PP at the interdesmosomal plasma membrane. Colocalizes with cortical actin-based membrane structures.
- Information by UniProt
- HRIHFB2003 antibody
- KAZ antibody
- Kazn antibody
All lanes : Anti-Kazrin antibody (ab74114) at 1 µg/ml
Lane 1 : Over Expressing HA tagged KazrinA
Lane 2 : Over Expressing HA tagged KazrinE
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 86 kDa
Observed band size: 100,53 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes
Several isoforms of Kazrin are expressed in human keratinoctyes. KazrinE is the longest isoform and has a predicted molecular weight of 86 kDa, however is expected to show a banding pattern around 100 kDa within western blotting. KazrinA is the most abundant isoform and has a predicted molecular weight of 47 kDa, however shows a banding pattern around 53 kDa on a western blot. Abcam recommends using milk (2%) as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
ICC/IF image of ab74114 stained SKNSH cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab74114, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab74114 has not yet been referenced specifically in any publications.