Anti-Kazrin antibody (ab88752)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Kazrin antibody
See all Kazrin primary antibodies -
Description
Mouse polyclonal to Kazrin -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Full length protein, corresponding to amino acids 1-415 of Human Kazrin (NP_001018000.1)
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Positive control
- Rat brain tissue lysate and transfected 293T cell lysate
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Constituent: 2.68% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab88752 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 86 kDa. |
Target
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Function
Component of the cornified envelope of keratinocytes. May be involved in the interplay between adherens junctions and desmosomes. The function in the nucleus is not known. -
Tissue specificity
Isoform 2, isoform 3 and isoform 4 are expressed in several cell lines including keratinocytes and bladder and epidermoid carcinoma (at protein level). Isoform 2, isoform 3 and isoform 4 are expressed in hair follicle and interfollicular epidermis (at protein level). -
Sequence similarities
Belongs to the kazrin family.
Contains 3 SAM (sterile alpha motif) domains. -
Cellular localization
Cytoplasm > cytoskeleton and Cytoplasm. Cell junction > desmosome. Nucleus. Observed at the apical plasma mebrane of keratinocytes. Partially colocalizes with PPL and DP at desmosomes, and with PP at the interdesmosomal plasma membrane. Colocalizes with cortical actin-based membrane structures. - Information by UniProt
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Database links
- Entrez Gene: 23254 Human
- Entrez Gene: 313672 Rat
- SwissProt: Q674X7 Human
- SwissProt: Q5FWS6 Rat
- Unigene: 368823 Human
- Unigene: 3753 Rat
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Alternative names
- HRIHFB2003 antibody
- KAZ antibody
- Kazn antibody
see all
Images
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Western blot - Anti-Kazrin antibody (ab88752)Anti-Kazrin antibody (ab88752) at 1 µg/ml + Rat brain tissue lysate at 50ug/lane
Predicted band size: 86 kDa
Observed band size: 86 kDa
Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands. -
Western blot - Anti-Kazrin antibody (ab88752)All lanes : Anti-Kazrin antibody (ab88752) at 1 µg/ml
Lane 1 : Kazrin transfected 293T cell lysate (45.65 kDa) at 25ug/lane
Lane 2 : Non-transfected 293T cell lysate at 25ug/lane
Predicted band size: 86 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
References
ab88752 has not yet been referenced specifically in any publications.
