Anti-Kazrin antibody (ab88752)
Key features and details
- Mouse polyclonal to Kazrin
- Suitable for: WB
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Kazrin antibody
See all Kazrin primary antibodies -
Description
Mouse polyclonal to Kazrin -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Full length protein, corresponding to amino acids 1-415 of Human Kazrin (NP_001018000.1)
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Positive control
- Rat brain tissue lysate and transfected 293T cell lysate
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.4
Constituent: 2.68% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab88752 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 86 kDa. |
Target
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Function
Component of the cornified envelope of keratinocytes. May be involved in the interplay between adherens junctions and desmosomes. The function in the nucleus is not known. -
Tissue specificity
Isoform 2, isoform 3 and isoform 4 are expressed in several cell lines including keratinocytes and bladder and epidermoid carcinoma (at protein level). Isoform 2, isoform 3 and isoform 4 are expressed in hair follicle and interfollicular epidermis (at protein level). -
Sequence similarities
Belongs to the kazrin family.
Contains 3 SAM (sterile alpha motif) domains. -
Cellular localization
Cytoplasm > cytoskeleton and Cytoplasm. Cell junction > desmosome. Nucleus. Observed at the apical plasma mebrane of keratinocytes. Partially colocalizes with PPL and DP at desmosomes, and with PP at the interdesmosomal plasma membrane. Colocalizes with cortical actin-based membrane structures. - Information by UniProt
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Database links
- Entrez Gene: 23254 Human
- Entrez Gene: 313672 Rat
- SwissProt: Q674X7 Human
- SwissProt: Q5FWS6 Rat
- Unigene: 368823 Human
- Unigene: 3753 Rat
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Alternative names
- HRIHFB2003 antibody
- KAZ antibody
- Kazn antibody
see all
Images
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Western blot - Anti-Kazrin antibody (ab88752)Anti-Kazrin antibody (ab88752) at 1 µg/ml + Rat brain tissue lysate at 50ug/lane
Predicted band size: 86 kDa
Observed band size: 86 kDa
Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands. -
Western blot - Anti-Kazrin antibody (ab88752)All lanes : Anti-Kazrin antibody (ab88752) at 1 µg/ml
Lane 1 : Kazrin transfected 293T cell lysate (45.65 kDa) at 25ug/lane
Lane 2 : Non-transfected 293T cell lysate at 25ug/lane
Predicted band size: 86 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Datasheets and documents
References (0)
ab88752 has not yet been referenced specifically in any publications.
