Key features and details
- Rabbit polyclonal to KBTB6/KBTBD6
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-KBTB6/KBTBD6 antibody
DescriptionRabbit polyclonal to KBTB6/KBTBD6
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
- WB: HeLa cell lysate; mouse liver, kidney and brain tissue lysates. IHC-P: Human liver tissue. ICC/IF: HeLa cells.
This product was previously labelled as KBTB6
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%
Our Abpromise guarantee covers the use of ab222971 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000. Detects a band of approximately 77, 50 kDa (predicted molecular weight: 76 kDa).|
|IHC-P||1/20 - 1/200.|
|ICC/IF||1/50 - 1/200.|
Sequence similaritiesContains 1 BTB (POZ) domain.
Contains 6 Kelch repeats.
- Information by UniProt
- KBTB6_HUMAN antibody
- KBTBD6 antibody
- Kelch repeat and BTB (POZ) domain containing 6 antibody
- Kelch repeat and BTB domain-containing protein 6 antibody
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for KBTB6/KBTBD6 (green) using ab222971 at 1/100 dilution in ICC/IF. Secondary: Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).
Paraffin-embedded human liver tissue stained for KBTB6/KBTBD6 using ab222971 at 1/100 dilution in immunohistochemical analysis.
All lanes : Anti-KBTB6/KBTBD6 antibody (ab222971) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : Mouse liver tissue lysate
Lane 3 : Mouse kidney tissue lysate
Lane 4 : Mouse brain tissue lysate
All lanes : Goat Anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 76 kDa
Observed band size: 50,77 kDa why is the actual band size different from the predicted?
ab222971 has not yet been referenced specifically in any publications.