Anti-KBTBD4 antibody (ab235357)
Key features and details
- Rabbit polyclonal to KBTBD4
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-KBTBD4 antibody
See all KBTBD4 primary antibodies -
Description
Rabbit polyclonal to KBTBD4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human KBTBD4 aa 151-415.
Database link: Q9NVX7 -
Positive control
- WB: U-87 MG and NIH/3T3 whole cell lysate. IHC-P: Human breast cancer and testis tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95% -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab235357 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/2000. Predicted molecular weight: 58 kDa.
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IHC-P |
1/20 - 1/200.
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Notes |
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WB
1/500 - 1/2000. Predicted molecular weight: 58 kDa. |
IHC-P
1/20 - 1/200. |
Target
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Sequence similarities
Contains 1 BACK (BTB/Kelch associated) domain.
Contains 1 BTB (POZ) domain.
Contains 5 Kelch repeats. - Information by UniProt
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Database links
- Entrez Gene: 55709 Human
- Entrez Gene: 67136 Mouse
- SwissProt: Q9NVX7 Human
- SwissProt: Q8R179 Mouse
- Unigene: 656205 Human
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Alternative names
- BKLHD4 antibody
- BTB and kelch domain containing 4 antibody
- BTB and kelch domain-containing protein 4 antibody
see all
Images
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Paraffin-embedded human breast cancer stained for KBTBD4 using ab235357 at 1/100 dilution in immunohistochemical analysis.
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Paraffin-embedded human testis stained for KBTBD4 using ab235357 at 1/100 dilution in immunohistochemical analysis.
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All lanes : Anti-KBTBD4 antibody (ab235357) at 1/500 dilution
Lane 1 : U-87 (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
Lane 2 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Secondary
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 58 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab235357 has not yet been referenced specifically in any publications.