Key features and details
- Rabbit polyclonal to KBTBD4
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-KBTBD4 antibody
See all KBTBD4 primary antibodies
DescriptionRabbit polyclonal to KBTBD4
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment corresponding to Human KBTBD4 aa 151-415.
Database link: Q9NVX7
- WB: U-87 MG and NIH/3T3 whole cell lysate. IHC-P: Human breast cancer and testis tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%
Our Abpromise guarantee covers the use of ab235357 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 58 kDa.|
|IHC-P||1/20 - 1/200.|
Sequence similaritiesContains 1 BACK (BTB/Kelch associated) domain.
Contains 1 BTB (POZ) domain.
Contains 5 Kelch repeats.
- Information by UniProt
- BKLHD4 antibody
- BTB and kelch domain containing 4 antibody
- BTB and kelch domain-containing protein 4 antibody
Paraffin-embedded human breast cancer stained for KBTBD4 using ab235357 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human testis stained for KBTBD4 using ab235357 at 1/100 dilution in immunohistochemical analysis.
All lanes : Anti-KBTBD4 antibody (ab235357) at 1/500 dilution
Lane 1 : U-87 (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
Lane 2 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 58 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab235357 has not yet been referenced specifically in any publications.