Anti-KCC1 antibody (ab115607)
Key features and details
- Goat polyclonal to KCC1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-KCC1 antibody -
Description
Goat polyclonal to KCC1 -
Host species
Goat -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Sheep, Rabbit, Horse, Cow, Dog, Pig -
Immunogen
Synthetic peptide corresponding to Human KCC1 aa 950-1050 (internal sequence) (Cysteine residue).
Database link: NP_005063.1 -
Positive control
- Human Placenta tissue; Human heart lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab115607 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 121 kDa.
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 121 kDa. |
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May contribute to cell volume homeostasis in single cells. May be involved in the regulation of basolateral Cl(-) exit in NaCl absorbing epithelia (By similarity). Isoform 4 has no transport activity. -
Tissue specificity
Ubiquitous. Levels are much higher in erythrocytes from patients with Hb SC and Hb SS compared to normal AA erythrocytes. This may contribute to red blood cell dehydration and to the manifestation of sickle cell disease by increasing the intracellular concentration of HbS. Isoform 1 was not detected in circulating reticulocytes. -
Sequence similarities
Belongs to the SLC12A transporter family. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 6560 Human
- Entrez Gene: 20498 Mouse
- Entrez Gene: 396992 Pig
- Entrez Gene: 29501 Rat
- Omim: 604119 Human
- SwissProt: Q9UP95 Human
- SwissProt: Q9JIS8 Mouse
- SwissProt: Q28677 Rabbit
see all -
Alternative names
- Electroneutral potassium chloride cotransporter 1 antibody
- Electroneutral potassium-chloride cotransporter 1 antibody
- Erythroid K Cl cotransporter 1 antibody
see all
Images
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ab115607, at 5µg/ml, staining KCC1 in Formalin-fixed, Paraffin-embedded Human Placenta tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
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Anti-KCC1 antibody (ab115607) at 0.3 µg/ml + Human heart lysate at 35 µg
Developed using the ECL technique.
Predicted band size: 121 kDa
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab115607 has been referenced in 1 publication.
- Steffensen AB et al. Cotransporter-mediated water transport underlying cerebrospinal fluid formation. Nat Commun 9:2167 (2018). PubMed: 29867199