Key features and details
- Rabbit polyclonal to KCC2
- Suitable for: ICC/IF, WB
- Reacts with: Rat
- Isotype: IgG
Product nameAnti-KCC2 antibody
See all KCC2 primary antibodies
DescriptionRabbit polyclonal to KCC2
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Rat
Predicted to work with: Mouse, Cow, Dog, Human
Synthetic peptide corresponding to Human KCC2 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in the following rat tissue lysates: brain; cortex; hippocampus.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab97502 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 126 kDa (predicted molecular weight: 126 kDa).|
FunctionMediates electroneutral potassium-chloride cotransport in mature neurons. Transport occurs under isotonic conditions, but is activated 20-fold by cell swelling. Important for Cl(-) homeostasis in neurons.
Tissue specificityBrain specific. Detected in neuronal cells.
Sequence similaritiesBelongs to the SLC12A transporter family.
- Information by UniProt
- Electroneutral potassium chloride cotransporter 2 antibody
- Electroneutral potassium-chloride cotransporter 2 antibody
- Erythroid K Cl cotransporter 2 antibody
All lanes : Anti-KCC2 antibody (ab97502) at 1 µg/ml
Lane 1 : Brain (Rat) Tissue Lysate
Lane 2 : Rat Cortex Tissue Lysate
Lane 3 : Rat Hippocampus Tissue Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 126 kDa
Observed band size: 126 kDa
Additional bands at: 250 kDa, 30 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
ICC/IF image of ab97502 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab97502, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab97502 has not yet been referenced specifically in any publications.