• Product name

  • Description

    Rabbit polyclonal to KCC2
  • Host species

  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Rat
    Predicted to work with: Mouse, Cow, Dog, Human
  • Immunogen

    Synthetic peptide corresponding to Human KCC2 aa 1-100 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab109638)

  • Positive control

    • This antibody gave a positive signal in the following rat tissue lysates: brain; cortex; hippocampus.



Our Abpromise guarantee covers the use of ab97502 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 126 kDa (predicted molecular weight: 126 kDa).


  • Function

    Mediates electroneutral potassium-chloride cotransport in mature neurons. Transport occurs under isotonic conditions, but is activated 20-fold by cell swelling. Important for Cl(-) homeostasis in neurons.
  • Tissue specificity

    Brain specific. Detected in neuronal cells.
  • Sequence similarities

    Belongs to the SLC12A transporter family.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • Electroneutral potassium chloride cotransporter 2 antibody
    • Electroneutral potassium-chloride cotransporter 2 antibody
    • Erythroid K Cl cotransporter 2 antibody
    • Furosemide sensitive K Cl cotransporter antibody
    • hKCC2 antibody
    • K-Cl cotransporter 2 antibody
    • KCC 2 antibody
    • KCC2 antibody
    • KIAA1176 antibody
    • Neuronal K Cl cotransporter antibody
    • Neuronal K-Cl cotransporter antibody
    • Potassium Chloride Cotransporter antibody
    • Potassium chloride transporter 5 antibody
    • rKCC2 antibody
    • S12A5 antibody
    • S12A5_HUMAN antibody
    • SLC12A5 antibody
    • Solute carrier family 12 (potassium chloride transporter) member 5 antibody
    • Solute carrier family 12 member 5 antibody
    see all


  • All lanes : Anti-KCC2 antibody (ab97502) at 1 µg/ml

    Lane 1 : Brain (Rat) Tissue Lysate
    Lane 2 : Rat Cortex Tissue Lysate
    Lane 3 : Rat Hippocampus Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 126 kDa
    Observed band size: 126 kDa
    Additional bands at: 250 kDa, 30 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 2 minutes
  • ICC/IF image of ab97502 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab97502, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


ab97502 has not yet been referenced specifically in any publications.

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