For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
I have to get an excellent identification of NKCC2 in inmunohistochemistry in frozen section of rat brain, but until now I did not get it with the antibody ab49917. Then I have though that maybe your another antibody ab134300 could be work better, but I am not sure, because you do not show any photograph about this in your page. May you help me respect my technician problems? Thank you!
Asked on Jul 13 2012
Thank you for contacting us.
Are you trying to detect NKCC2 or KCC2?
NKCC2 (SLC12a1) moves one sodium ion, one potassium ion and 2 chloride ions together. KCC2 (SLC12a5) moves two potassiums with 2 chlorides. Because of the differences in electrochemical driving force between K (outward) and Na (inward), KCC2 generally carries solute from inside the cell to outside, whereas NKCC2 generally carries solute into the cell. NKCC2 is basically specific to a single cell type in kidney. KCC2 is found in many cells. You mentioned NKCC2 not KCC2, although some people do use that acronym for KCC2 as well.
The target of antibodies ab49117 and ab134300 is described at the following URL:
Answered on Jul 13 2012