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    kcc2-antibody-n166-ab174936.pdf

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Neuroscience Neurotransmission Receptors / Channels Potassium Channels
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Anti-KCC2 antibody [N1/66] (ab174936)

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Western blot - Anti-KCC2 antibody [N1/66] (ab174936)

    Key features and details

    • Mouse monoclonal [N1/66] to KCC2
    • Suitable for: WB
    • Reacts with: Rat
    • Isotype: IgG1

    You may also be interested in

    Secondary
    Product image
    Goat Anti-Mouse IgG H&L (HRP) (ab205719)

    View more associated products

    Overview

    • Product name

      Anti-KCC2 antibody [N1/66]
      See all KCC2 primary antibodies
    • Description

      Mouse monoclonal [N1/66] to KCC2
    • Host species

      Mouse
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Rat
      Predicted to work with: Mouse, Human
    • Immunogen

      Fusion protein corresponding to Rat KCC2. Amino acids 932-1043 (intracellular carboxyl terminus) of rat KCC2b.

    • Positive control

      • This antibody gave a positive signal in Western Blot against Rat cortex tissue lysate.
    • General notes

      This antibody clone is manufactured by Abcam.

      If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituents: PBS, 6.97% L-Arginine
    • Concentration information loading...
    • Purity

      Protein G purified
    • Clonality

      Monoclonal
    • Clone number

      N1/66
    • Myeloma

      Sp2/0
    • Isotype

      IgG1
    • Research areas

      • Neuroscience
      • Neurotransmission
      • Receptors / Channels
      • Potassium Channels
      • Neuroscience
      • Neurotransmission
      • Receptors / Channels
      • More Ion Channels

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

    Applications

    Our Abpromise guarantee covers the use of ab174936 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB Use a concentration of 5 µg/ml. Detects a band of approximately 140 kDa (predicted molecular weight: 126 kDa).

    Target

    • Function

      Mediates electroneutral potassium-chloride cotransport in mature neurons. Transport occurs under isotonic conditions, but is activated 20-fold by cell swelling. Important for Cl(-) homeostasis in neurons.
    • Tissue specificity

      Brain specific. Detected in neuronal cells.
    • Sequence similarities

      Belongs to the SLC12A transporter family.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession Q9H2X9 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 57468 Human
      • Entrez Gene: 57138 Mouse
      • Entrez Gene: 171373 Rat
      • Omim: 606726 Human
      • SwissProt: Q9H2X9 Human
      • SwissProt: Q91V14 Mouse
      • SwissProt: Q63633 Rat
      • Unigene: 21413 Human
      • Unigene: 252987 Mouse
      • Unigene: 10513 Rat
      see all
    • Alternative names

      • Electroneutral potassium chloride cotransporter 2 antibody
      • Electroneutral potassium-chloride cotransporter 2 antibody
      • Erythroid K Cl cotransporter 2 antibody
      • Furosemide sensitive K Cl cotransporter antibody
      • hKCC2 antibody
      • K-Cl cotransporter 2 antibody
      • KCC 2 antibody
      • KCC2 antibody
      • KIAA1176 antibody
      • Neuronal K Cl cotransporter antibody
      • Neuronal K-Cl cotransporter antibody
      • Potassium Chloride Cotransporter antibody
      • Potassium chloride transporter 5 antibody
      • rKCC2 antibody
      • S12A5 antibody
      • S12A5_HUMAN antibody
      • SLC12A5 antibody
      • Solute carrier family 12 (potassium chloride transporter) member 5 antibody
      • Solute carrier family 12 member 5 antibody
      see all

    Images

    • Western blot - Anti-KCC2 antibody [N1/66] (ab174936)
      Western blot - Anti-KCC2 antibody [N1/66] (ab174936)
      Anti-KCC2 antibody [N1/66] (ab174936) at 5 µg/ml + Rat Cortex Tissue Lysate at 20 µg

      Secondary
      Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size: 126 kDa
      Observed band size: 140 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 15 kDa (possible non-specific binding), 35 kDa (possible non-specific binding), 45 kDa (possible non-specific binding)


      Exposure time: 20 minutes

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab174936? Please let us know so that we can cite the reference in this datasheet.

    ab174936 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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