Key features and details
- Mouse monoclonal [N1/66] to KCC2
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG1
Product nameAnti-KCC2 antibody [N1/66]
See all KCC2 primary antibodies
DescriptionMouse monoclonal [N1/66] to KCC2
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Rat
Predicted to work with: Mouse, Human
Fusion protein corresponding to Rat KCC2. Amino acids 932-1043 (intracellular carboxyl terminus) of rat KCC2b.
- This antibody gave a positive signal in Western Blot against Rat cortex tissue lysate.
This antibody clone is manufactured by Abcam.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab174936 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 5 µg/ml. Detects a band of approximately 140 kDa (predicted molecular weight: 126 kDa).|
FunctionMediates electroneutral potassium-chloride cotransport in mature neurons. Transport occurs under isotonic conditions, but is activated 20-fold by cell swelling. Important for Cl(-) homeostasis in neurons.
Tissue specificityBrain specific. Detected in neuronal cells.
Sequence similaritiesBelongs to the SLC12A transporter family.
- Information by UniProt
- Electroneutral potassium chloride cotransporter 2 antibody
- Electroneutral potassium-chloride cotransporter 2 antibody
- Erythroid K Cl cotransporter 2 antibody
Anti-KCC2 antibody [N1/66] (ab174936) at 5 µg/ml + Rat Cortex Tissue Lysate at 20 µg
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 126 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?
Additional bands at: 15 kDa (possible non-specific binding), 35 kDa (possible non-specific binding), 45 kDa (possible non-specific binding)
Exposure time: 20 minutes
ab174936 has not yet been referenced specifically in any publications.