Anti-KCC2 antibody [N1/66] (ab174936)
![Western blot - Anti-KCC2 antibody [N1/66] (ab174936)](https://www.abcam.com/ps/products/174/ab174936/Images/ab174936-209735-anti-kcc2-antibody-n1-66-western-blot.jpg "Western blot - Anti-KCC2 antibody [N1/66] (ab174936)")
Key features and details
- Mouse monoclonal [N1/66] to KCC2
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG1
Overview
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Product name
Anti-KCC2 antibody [N1/66]
See all KCC2 primary antibodies -
Description
Mouse monoclonal [N1/66] to KCC2 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Rat
Predicted to work with: Mouse, Human
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Immunogen
Fusion protein corresponding to Rat KCC2. Amino acids 932-1043 (intracellular carboxyl terminus) of rat KCC2b.
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Positive control
- This antibody gave a positive signal in Western Blot against Rat cortex tissue lysate.
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General notes
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
N1/66 -
Myeloma
Sp2/0 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab174936 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 5 µg/ml. Detects a band of approximately 140 kDa (predicted molecular weight: 126 kDa). |
Target
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Function
Mediates electroneutral potassium-chloride cotransport in mature neurons. Transport occurs under isotonic conditions, but is activated 20-fold by cell swelling. Important for Cl(-) homeostasis in neurons. -
Tissue specificity
Brain specific. Detected in neuronal cells. -
Sequence similarities
Belongs to the SLC12A transporter family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 57468 Human
- Entrez Gene: 57138 Mouse
- Entrez Gene: 171373 Rat
- Omim: 606726 Human
- SwissProt: Q9H2X9 Human
- SwissProt: Q91V14 Mouse
- SwissProt: Q63633 Rat
- Unigene: 21413 Human
see all -
Alternative names
- Electroneutral potassium chloride cotransporter 2 antibody
- Electroneutral potassium-chloride cotransporter 2 antibody
- Erythroid K Cl cotransporter 2 antibody
see all
Images
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Western blot - Anti-KCC2 antibody [N1/66] (ab174936)Anti-KCC2 antibody [N1/66] (ab174936) at 5 µg/ml + Rat Cortex Tissue Lysate at 20 µg
Secondary
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 126 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?
Additional bands at: 15 kDa (possible non-specific binding), 35 kDa (possible non-specific binding), 45 kDa (possible non-specific binding)
Exposure time: 20 minutes
Datasheets and documents
References (0)
ab174936 has not yet been referenced specifically in any publications.
