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Mouse monoclonal [N1/66] to KCC2
100% identity with KCC2a.
<40% identity with KCC1, KCC3, KCC4.
Predicted to work with:
Fusion protein corresponding to Rat KCC2. Amino acids 932-1043 (intracellular carboxyl terminus) of rat
This antibody gave a positive signal in Western Blot against Rat cortex tissue lysate.
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact
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Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide Constituents: PBS, 6.97% L-Arginine
Concentration information loading...
Protein G purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml. Detects a band of approximately 140 kDa (predicted molecular weight: 126 kDa).
Mediates electroneutral potassium-chloride cotransport in mature neurons. Transport occurs under isotonic conditions, but is activated 20-fold by cell swelling. Important for Cl(-) homeostasis in neurons.
Brain specific. Detected in neuronal cells.
Belongs to the SLC12A transporter family.
Information by UniProt
Electroneutral potassium chloride cotransporter 2 antibody
Electroneutral potassium-chloride cotransporter 2 antibody
Erythroid K Cl cotransporter 2 antibody
Western blot - Anti-KCC2 antibody [N1/66] (ab174936)
Anti-KCC2 antibody [N1/66] (ab174936) at 5 µg/ml + Rat Cortex Tissue Lysate at 20 µg
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (
) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size:
Observed band size:
140 kDa (
why is the actual band size different from the predicted?
Additional bands at:
15 kDa (possible non-specific binding), 35 kDa (possible non-specific binding), 45 kDa (possible non-specific binding)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"