Overview

  • Product name
    Anti-KCC2 antibody [N1/66]
    See all KCC2 primary antibodies
  • Description
    Mouse monoclonal [N1/66] to KCC2
  • Host species
    Mouse
  • Specificity
    100% identity with KCC2a. <40% identity with KCC1, KCC3, KCC4.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Rat
    Predicted to work with: Mouse, Human
  • Immunogen

    Fusion protein corresponding to Rat KCC2. Amino acids 932-1043 (intracellular carboxyl terminus) of rat KCC2b.

  • Positive control
    • This antibody gave a positive signal in Western Blot against Rat cortex tissue lysate.
  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Applications

Our Abpromise guarantee covers the use of ab174936 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 5 µg/ml. Detects a band of approximately 140 kDa (predicted molecular weight: 126 kDa).

Target

  • Function
    Mediates electroneutral potassium-chloride cotransport in mature neurons. Transport occurs under isotonic conditions, but is activated 20-fold by cell swelling. Important for Cl(-) homeostasis in neurons.
  • Tissue specificity
    Brain specific. Detected in neuronal cells.
  • Sequence similarities
    Belongs to the SLC12A transporter family.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • Electroneutral potassium chloride cotransporter 2 antibody
    • Electroneutral potassium-chloride cotransporter 2 antibody
    • Erythroid K Cl cotransporter 2 antibody
    • Furosemide sensitive K Cl cotransporter antibody
    • hKCC2 antibody
    • K-Cl cotransporter 2 antibody
    • KCC 2 antibody
    • KCC2 antibody
    • KIAA1176 antibody
    • Neuronal K Cl cotransporter antibody
    • Neuronal K-Cl cotransporter antibody
    • Potassium Chloride Cotransporter antibody
    • Potassium chloride transporter 5 antibody
    • rKCC2 antibody
    • S12A5 antibody
    • S12A5_HUMAN antibody
    • SLC12A5 antibody
    • Solute carrier family 12 (potassium chloride transporter) member 5 antibody
    • Solute carrier family 12 member 5 antibody
    see all

Images

  • Western blot - Anti-KCC2 antibody [N1/66] (ab174936)
    Western blot - Anti-KCC2 antibody [N1/66] (ab174936)
    Anti-KCC2 antibody [N1/66] (ab174936) at 5 µg/ml + Rat Cortex Tissue Lysate at 20 µg

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 126 kDa
    Observed band size: 140 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 15 kDa (possible non-specific binding), 35 kDa (possible non-specific binding), 45 kDa (possible non-specific binding)


    Exposure time: 20 minutes

References

ab174936 has not yet been referenced specifically in any publications.

Publishing research using ab174936? Please let us know so that we can cite the reference in this datasheet.

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