Anti-KCC2 antibody [N1/66] (ab174936)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-KCC2 antibody [N1/66]
See all KCC2 primary antibodies -
DescriptionMouse monoclonal [N1/66] to KCC2
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Host speciesMouse
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Specificity100% identity with KCC2a. <40% identity with KCC1, KCC3, KCC4.
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Tested applicationsSuitable for: WBmore details
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Species reactivityReacts with: Rat
Predicted to work with: Mouse, Human
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Immunogen
Fusion protein corresponding to Rat KCC2. Amino acids 932-1043 (intracellular carboxyl terminus) of rat KCC2b.
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Positive control
- This antibody gave a positive signal in Western Blot against Rat cortex tissue lysate.
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General notes
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
PurityProtein G purified
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ClonalityMonoclonal
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Clone numberN1/66
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MyelomaSp2/0
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IsotypeIgG1
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab174936 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 5 µg/ml. Detects a band of approximately 140 kDa (predicted molecular weight: 126 kDa). |
Target
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FunctionMediates electroneutral potassium-chloride cotransport in mature neurons. Transport occurs under isotonic conditions, but is activated 20-fold by cell swelling. Important for Cl(-) homeostasis in neurons.
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Tissue specificityBrain specific. Detected in neuronal cells.
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Sequence similaritiesBelongs to the SLC12A transporter family.
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Cellular localizationMembrane.
- Information by UniProt
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Database links
- Entrez Gene: 57468 Human
- Entrez Gene: 57138 Mouse
- Entrez Gene: 171373 Rat
- Omim: 606726 Human
- SwissProt: Q9H2X9 Human
- SwissProt: Q91V14 Mouse
- SwissProt: Q63633 Rat
- Unigene: 21413 Human
see all -
Alternative names
- Electroneutral potassium chloride cotransporter 2 antibody
- Electroneutral potassium-chloride cotransporter 2 antibody
- Erythroid K Cl cotransporter 2 antibody
see all
Images
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![Western blot - Anti-KCC2 antibody [N1/66] (ab174936)](https://www.abcam.com/ps/products/174/ab174936/Images/ab174936-209735-WBAP16934511.jpg)
Western blot - Anti-KCC2 antibody [N1/66] (ab174936)Anti-KCC2 antibody [N1/66] (ab174936) at 5 µg/ml + Rat Cortex Tissue Lysate at 20 µg
Secondary
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 126 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?
Additional bands at: 15 kDa (possible non-specific binding), 35 kDa (possible non-specific binding), 45 kDa (possible non-specific binding)
Exposure time: 20 minutes
Protocols
Datasheets and documents
References
ab174936 has not yet been referenced specifically in any publications.
