Product nameAnti-KCHIP1 antibody [EPR7624]
See all KCHIP1 primary antibodies
DescriptionRabbit monoclonal [EPR7624] to KCHIP1
Tested applicationsSuitable for: WB, IP, Flow Cytmore details
Unsuitable for: ICC or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
corresponding to Human KCHIP1 aa 50-150.
Database link: Q9NZI2
- Human small intestine lysate, Human fetal heart lysate, BxPC3 cell lysate, Caco2 cell lysate, HepG2 cells and cell lysate.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 5.00 x 10 -12 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab133753 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 30 kDa (predicted molecular weight: 26 kDa).|
|IP||1/10 - 1/100.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionRegulatory subunit of Kv4/D (Shal)-type voltage-gated rapidly inactivating A-type potassium channels. Regulates channel density, inactivation kinetics and rate of recovery from inactivation in a calcium-dependent and isoform-specific manner. In vitro, modulates KCND1/Kv4.1 and KCND2/Kv4.2 currents. Increases the presence of KCND2 at the cell surface.
Tissue specificityIsoform 1 and isoform 2 are expressed in brain and kidney. Isoform 1 is also expressed in liver, pancreas, skeletal muscle, small intestine and testis. Isoform 2 is also expressed in lung, pancreas, leukocytes, prostate and thymus.
Sequence similaritiesBelongs to the recoverin family.
Contains 4 EF-hand domains.
Cellular localizationCell membrane. Cytoplasm. Cell projection, dendrite.
- Information by UniProt
FormThere are 3 isoforms produced by alternative splicing.
- A type potassium channel modulatory protein 1 antibody
- A-type potassium channel modulatory protein 1 antibody
- KChIP1 antibody
All lanes : Anti-KCHIP1 antibody [EPR7624] (ab133753) at 1/1000 dilution
Lane 1 : Human small intestine lysate
Lane 2 : Human fetal heart lysate
Lane 3 : BxPC3 cell lysate
Lane 4 : Caco2 cell lysate
Lane 5 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti Rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 26 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
Flow cytometric analysis of permeabilized HepG2 cells labelling KCHIP1 with ab133753 at 1/10 (red) or a rabbit IgG negative control antibody (green).
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab133753 has not yet been referenced specifically in any publications.