Recombinant
RabMAb

Recombinant Anti-KCHIP1 antibody [EPR7624] (ab133753)

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Overview

  • Product name

    Anti-KCHIP1 antibody [EPR7624]
    See all KCHIP1 primary antibodies

  • Description

    Rabbit monoclonal [EPR7624] to KCHIP1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, Flow Cytmore details
    Unsuitable for: ICC or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    corresponding to Human KCHIP1 aa 50-150.
    Database link: Q9NZI2

  • Positive control

    • Human small intestine lysate, Human fetal heart lysate, BxPC3 cell lysate, Caco2 cell lysate, HepG2 cells and cell lysate.

  • General notes

     

     

     This product was previously labelled as KCNIP1

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Dissociation constant (KD)

    KD = 5.00 x 10 -12 M
    Learn more about KD

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR7624

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab133753 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 30 kDa (predicted molecular weight: 26 kDa).
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for ICC or IHC-P.

    • Target

    • Function

      Regulatory subunit of Kv4/D (Shal)-type voltage-gated rapidly inactivating A-type potassium channels. Regulates channel density, inactivation kinetics and rate of recovery from inactivation in a calcium-dependent and isoform-specific manner. In vitro, modulates KCND1/Kv4.1 and KCND2/Kv4.2 currents. Increases the presence of KCND2 at the cell surface.

    • Tissue specificity

      Isoform 1 and isoform 2 are expressed in brain and kidney. Isoform 1 is also expressed in liver, pancreas, skeletal muscle, small intestine and testis. Isoform 2 is also expressed in lung, pancreas, leukocytes, prostate and thymus.

    • Sequence similarities

      Belongs to the recoverin family.
      Contains 4 EF-hand domains.

    • Cellular localization

      Cell membrane. Cytoplasm. Cell projection, dendrite.
    • Information by UniProt

    • Database links

      see all

    • Form

      There are 3 isoforms produced by alternative splicing.

    • Alternative names

      • A type potassium channel modulatory protein 1 antibody
      • A-type potassium channel modulatory protein 1 antibody
      • KChIP1 antibody
      • KCIP1_HUMAN antibody
      • Kcnip1 antibody
      • Kv channel interacting protein 1 antibody
      • Kv channel-interacting protein 1 antibody
      • MGC9 antibody
      • MGC95 antibody
      • Potassium channel interacting protein 1 antibody
      • Potassium channel-interacting protein 1 antibody
      • VABP antibody
      • Vesicle APC binding protein antibody
      • Vesicle APC-binding protein antibody
      see all

    Images

    • Western blot - Anti-KCHIP1 antibody [EPR7624] (ab133753)
      Western blot - Anti-KCHIP1 antibody [EPR7624] (ab133753)
      All lanes : Anti-KCHIP1 antibody [EPR7624] (ab133753) at 1/1000 dilution

      Lane 1 : Human small intestine lysate
      Lane 2 : Human fetal heart lysate
      Lane 3 : BxPC3 cell lysate
      Lane 4 : Caco2 cell lysate
      Lane 5 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti Rabbit HRP conjugated antibody at 1/2000 dilution

      Predicted band size: 26 kDa
      Observed band size: 30 kDa
      why is the actual band size different from the predicted?

    • Flow Cytometry - Anti-KCHIP1 antibody [EPR7624] (ab133753)
      Flow Cytometry - Anti-KCHIP1 antibody [EPR7624] (ab133753)

      Flow cytometric analysis of permeabilized HepG2 cells labelling KCHIP1 with ab133753 at 1/10 (red) or a rabbit IgG negative control antibody (green).

    • Other - Anti-KCHIP1 antibody [EPR7624] (ab133753)
      Other - Anti-KCHIP1 antibody [EPR7624] (ab133753)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab133753 has not yet been referenced specifically in any publications.

    Publishing research using ab133753? Please let us know so that we can cite the reference in this datasheet.

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