Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7624] to KCHIP1
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Rat, Human
Product nameAnti-KCHIP1 antibody [EPR7624]
See all KCHIP1 primary antibodies
DescriptionRabbit monoclonal [EPR7624] to KCHIP1
Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: ICC or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
corresponding to Human KCHIP1 aa 50-150.
Database link: Q9NZI2
- Human small intestine lysate, Human fetal heart lysate, BxPC3 cell lysate, Caco2 cell lysate, HepG2 cells and cell lysate.
This product was previously labelled as KCNIP1
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 5.00 x 10 -12 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab133753 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 30 kDa (predicted molecular weight: 26 kDa).|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionRegulatory subunit of Kv4/D (Shal)-type voltage-gated rapidly inactivating A-type potassium channels. Regulates channel density, inactivation kinetics and rate of recovery from inactivation in a calcium-dependent and isoform-specific manner. In vitro, modulates KCND1/Kv4.1 and KCND2/Kv4.2 currents. Increases the presence of KCND2 at the cell surface.
Tissue specificityIsoform 1 and isoform 2 are expressed in brain and kidney. Isoform 1 is also expressed in liver, pancreas, skeletal muscle, small intestine and testis. Isoform 2 is also expressed in lung, pancreas, leukocytes, prostate and thymus.
Sequence similaritiesBelongs to the recoverin family.
Contains 4 EF-hand domains.
Cellular localizationCell membrane. Cytoplasm. Cell projection, dendrite.
- Information by UniProt
FormThere are 3 isoforms produced by alternative splicing.
- A type potassium channel modulatory protein 1 antibody
- A-type potassium channel modulatory protein 1 antibody
- KChIP1 antibody
All lanes : Anti-KCHIP1 antibody [EPR7624] (ab133753) at 1/1000 dilution
Lane 1 : Human small intestine lysate
Lane 2 : Human fetal heart lysate
Lane 3 : BxPC3 cell lysate
Lane 4 : Caco2 cell lysate
Lane 5 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti Rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 26 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
Flow cytometric analysis of permeabilized HepG2 cells labelling KCHIP1 with ab133753 at 1/10 (red) or a rabbit IgG negative control antibody (green).
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab133753 has not yet been referenced specifically in any publications.