Recombinant Anti-KCHIP1 antibody [EPR7624] - BSA and Azide free (ab248639)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7624] to KCHIP1 - BSA and Azide free
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-KCHIP1 antibody [EPR7624] - BSA and Azide free
See all KCHIP1 primary antibodies -
Description
Rabbit monoclonal [EPR7624] to KCHIP1 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
Application Species Flow Cyt HumanWB Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab248639 is the carrier-free version of ab133753 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab248639 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as KCNIP1
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Dissociation constant (KD)
KD = 5.00 x 10 -12 M Learn more about KD -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR7624 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab248639 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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Flow Cyt |
Human
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WB |
Human
|
Application | Abreviews | Notes |
---|---|---|
WB |
Use at an assay dependent concentration. Detects a band of approximately 30 kDa (predicted molecular weight: 26 kDa).
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|
Flow Cyt |
Use at an assay dependent concentration.
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Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 30 kDa (predicted molecular weight: 26 kDa). |
Flow Cyt
Use at an assay dependent concentration. |
Target
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Function
Regulatory subunit of Kv4/D (Shal)-type voltage-gated rapidly inactivating A-type potassium channels. Regulates channel density, inactivation kinetics and rate of recovery from inactivation in a calcium-dependent and isoform-specific manner. In vitro, modulates KCND1/Kv4.1 and KCND2/Kv4.2 currents. Increases the presence of KCND2 at the cell surface. -
Tissue specificity
Isoform 1 and isoform 2 are expressed in brain and kidney. Isoform 1 is also expressed in liver, pancreas, skeletal muscle, small intestine and testis. Isoform 2 is also expressed in lung, pancreas, leukocytes, prostate and thymus. -
Sequence similarities
Belongs to the recoverin family.
Contains 4 EF-hand domains. -
Cellular localization
Cell membrane. Cytoplasm. Cell projection, dendrite. - Information by UniProt
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Database links
- Entrez Gene: 30820 Human
- Entrez Gene: 70357 Mouse
- Entrez Gene: 65023 Rat
- Omim: 604660 Human
- SwissProt: Q9NZI2 Human
- SwissProt: Q9JJ57 Mouse
- SwissProt: Q8R426 Rat
- Unigene: 484111 Human
see all -
Form
There are 3 isoforms produced by alternative splicing. -
Alternative names
- A type potassium channel modulatory protein 1 antibody
- A-type potassium channel modulatory protein 1 antibody
- KChIP1 antibody
see all
Images
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All lanes : Anti-KCHIP1 antibody [EPR7624] (ab133753) at 1/1000 dilution
Lane 1 : Human small intestine lysate
Lane 2 : Human fetal heart tissue lysate
Lane 3 : BxPC-3 cell lysate
Lane 4 : Caco-2 cell lysate
Lane 5 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti Rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 26 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?This data was developed using ab133753, the same antibody clone in a different buffer formulation.
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This data was developed using ab133753, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab248639 has not yet been referenced specifically in any publications.