RecombinantRabMAb

Recombinant Anti-KCHIP1 antibody [EPR7624] - BSA and Azide free (ab248639)

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Western blot - Anti-KCHIP1 antibody [EPR7624] - BSA and Azide free (ab248639)
  • Flow Cytometry - Anti-KCHIP1 antibody [EPR7624] - BSA and Azide free (ab248639)
  • OI-RD Scanning - Anti-KCHIP1 antibody [EPR7624] - BSA and Azide free (ab248639)
  • Anti-KCHIP1 antibody [EPR7624] - BSA and Azide free (ab248639)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7624] to KCHIP1 - BSA and Azide free
  • Suitable for: WB, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-KCHIP1 antibody [EPR7624] - BSA and Azide free
    See all KCHIP1 primary antibodies

  • Description

    Rabbit monoclonal [EPR7624] to KCHIP1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab248639 is the carrier-free version of ab133753 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab248639 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as KCNIP1

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Dissociation constant (KD)

    KD = 5.00 x 10 -12 M
    Learn more about KD

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Affinity purified

  • Clonality

    Monoclonal

  • Clone number

    EPR7624

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab248639 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
WB
Human
Application Abreviews Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 30 kDa (predicted molecular weight: 26 kDa).
Flow Cyt
Use at an assay dependent concentration.
Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 30 kDa (predicted molecular weight: 26 kDa).
Flow Cyt
Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for ICC or IHC-P.

    • Target

    • Function

      Regulatory subunit of Kv4/D (Shal)-type voltage-gated rapidly inactivating A-type potassium channels. Regulates channel density, inactivation kinetics and rate of recovery from inactivation in a calcium-dependent and isoform-specific manner. In vitro, modulates KCND1/Kv4.1 and KCND2/Kv4.2 currents. Increases the presence of KCND2 at the cell surface.

    • Tissue specificity

      Isoform 1 and isoform 2 are expressed in brain and kidney. Isoform 1 is also expressed in liver, pancreas, skeletal muscle, small intestine and testis. Isoform 2 is also expressed in lung, pancreas, leukocytes, prostate and thymus.

    • Sequence similarities

      Belongs to the recoverin family.
      Contains 4 EF-hand domains.

    • Cellular localization

      Cell membrane. Cytoplasm. Cell projection, dendrite.
    • Information by UniProt

    • Database links

      see all

    • Form

      There are 3 isoforms produced by alternative splicing.

    • Alternative names

      • A type potassium channel modulatory protein 1 antibody
      • A-type potassium channel modulatory protein 1 antibody
      • KChIP1 antibody
      • KCIP1_HUMAN antibody
      • Kcnip1 antibody
      • Kv channel interacting protein 1 antibody
      • Kv channel-interacting protein 1 antibody
      • MGC9 antibody
      • MGC95 antibody
      • Potassium channel interacting protein 1 antibody
      • Potassium channel-interacting protein 1 antibody
      • VABP antibody
      • Vesicle APC binding protein antibody
      • Vesicle APC-binding protein antibody
      see all

    Images

    • Western blot - Anti-KCHIP1 antibody [EPR7624] - BSA and Azide free (ab248639)
      Western blot - Anti-KCHIP1 antibody [EPR7624] - BSA and Azide free (ab248639)
      All lanes : Anti-KCHIP1 antibody [EPR7624] (ab133753) at 1/1000 dilution

      Lane 1 : Human small intestine lysate
      Lane 2 : Human fetal heart tissue lysate
      Lane 3 : BxPC-3 cell lysate
      Lane 4 : Caco-2 cell lysate
      Lane 5 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti Rabbit HRP conjugated antibody at 1/2000 dilution

      Predicted band size: 26 kDa
      Observed band size: 30 kDa
      why is the actual band size different from the predicted?



      This data was developed using ab133753, the same antibody clone in a different buffer formulation.

    • Flow Cytometry - Anti-KCHIP1 antibody [EPR7624] - BSA and Azide free (ab248639)
      Flow Cytometry - Anti-KCHIP1 antibody [EPR7624] - BSA and Azide free (ab248639)
      This data was developed using ab133753, the same antibody clone in a different buffer formulation.Flow cytometric analysis of permeabilized HepG2 cells labelling KCHIP1 with ab133753 at 1/10 (red) or a rabbit IgG negative control antibody (green).
    • OI-RD Scanning - Anti-KCHIP1 antibody [EPR7624] - BSA and Azide free (ab248639)
      OI-RD Scanning - Anti-KCHIP1 antibody [EPR7624] - BSA and Azide free (ab248639)
      This data was developed using ab133753, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-KCHIP1 antibody [EPR7624] - BSA and Azide free (ab248639)
      Anti-KCHIP1 antibody [EPR7624] - BSA and Azide free (ab248639)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab248639? Please let us know so that we can cite the reference in this datasheet.

    ab248639 has not yet been referenced specifically in any publications.

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