Recombinant Anti-KChIP2 antibody [K60/73] (ab252539)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [K60/73] to KChIP2
- Suitable for: IHC-P
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
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Product name
Anti-KChIP2 antibody [K60/73]
See all KChIP2 primary antibodies -
Description
Mouse monoclonal [K60/73] to KChIP2 -
Host species
Mouse -
Tested applications
Suitable for: IHC-Pmore details
Unsuitable for: ICC/IF or WB -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant fragment within Rat KChIP2 aa 1-300. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q9JM59 -
Positive control
- IHC-P: Mouse and rat cerebrum tissue.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
K60/73 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab252539 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/100.
|
Notes |
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IHC-P
1/100. |
Target
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Function
Regulatory subunit of Kv4/D (Shal)-type voltage-gated rapidly inactivating A-type potassium channels. Modulates channel density, inactivation kinetics and rate of recovery from inactivation in a calcium-dependent and isoform-specific manner. In vitro, modulates KCND2/Kv4.2 and KCND3/Kv4.3 currents. Involved in KCND2 and KCND3 trafficking to the cell surface. May be required for the expression of I(To) currents in the heart. -
Tissue specificity
Expressed in brain. Colocalizes with KCND2 in excitatory neurons including cortical and hippocampal CA1 pyramidal cells. Isoform 3 is expressed in heart and in umbilical vein endothelial cells. Not expressed in fetal heart. -
Sequence similarities
Belongs to the recoverin family.
Contains 4 EF-hand domains. -
Post-translational
modificationsPalmitoylated. Palmitoylation enhances association with the plasma membrane. -
Cellular localization
Cell membrane and Cell membrane. Detected on lipid rafts (By similarity). - Information by UniProt
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Database links
- Entrez Gene: 80906 Mouse
- Entrez Gene: 100911951 Rat
- Entrez Gene: 56817 Rat
- SwissProt: Q9JJ69 Mouse
- SwissProt: Q9JM59 Rat
- Unigene: 213204 Mouse
- Unigene: 98384 Rat
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Form
There are 9 isoforms produced by alternative splicing. -
Alternative names
- A type potassium channel modulatory protein 2 antibody
- A-type potassium channel modulatory protein 2 antibody
- Cardiac voltage gated potassium channel modulatory subunit antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling KChIP2 with ab252539 at 1/100 dilution followed by a ready to use secondary. Positive staining on the mouse cerebrum is observed. The section was incubated with ab252539 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use secondary.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling KChIP2 with ab252539 at 1/100 dilution followed by a ready to use secondary. Positive staining on the rat cerebrum is observed. The section was incubated with ab252539 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab252539 has not yet been referenced specifically in any publications.