Product nameKChIP2 overexpression 293T lysate (whole cell)
ab94274 is a 293T cell transfected lysate in which Human KChIP2 has been transiently over-expressed using a pCMV-KChIP2 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferConstituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine)
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BackgroundFunction: Regulatory subunit of Kv4/D (Shal)-type voltage-gated rapidly inactivating A-type potassium channels. Modulates channel density, inactivation kinetics and rate of recovery from inactivation in a calcium-dependent and isoform-specific manner. In vitro, modulates KCND2/Kv4.2 and KCND3/Kv4.3 currents. Involved in KCND2 and KCND3 trafficking to the cell surface. May be required for the expression of I(To) currents in the heart. PTM: Palmitoylated. Palmitoylation enhances association with the plasma membrane. Similarity: Belongs to the recoverin family. Contains 4 EF-hand domains. Tissue specificity: Expressed in brain. Colocalizes with KCND2 in excitatory neurons including cortical and hippocampal CA1 pyramidal cells. Isoform 3 is expressed in heart and in umbilical vein endothelial cells. Not expressed in fetal heart.
Our Abpromise guarantee covers the use of ab94274 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94274 at 15µg/lane on an SDS-PAGE gel.
All lanes : Anti-KChIP2 antibody (ab57207) at 1/500 dilution
Lane 1 :
KChIP2 overexpression 293T lysate (whole cell) (ab94274)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
ab94274 has not yet been referenced specifically in any publications.