Anti-KCNC1 antibody (ab254906)
Key features and details
- Rabbit polyclonal to KCNC1
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-KCNC1 antibody
See all KCNC1 primary antibodies -
Description
Rabbit polyclonal to KCNC1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment corresponding to Human KCNC1 aa 100-200.
Database link: P48547 -
Positive control
- ICC/IF: U-2 OS cells. IHC-P: Human cerebellum and cerebral cortex tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab254906 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
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IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient. Can form functional homotetrameric channels and heterotetrameric channels that contain variable proportions of KCNC2, and possibly other family members as well. Contributes to fire sustained trains of very brief action potentials at high frequency in pallidal neurons. -
Involvement in disease
Epilepsy, progressive myoclonic 7 -
Sequence similarities
Belongs to the potassium channel family. C (Shaw) (TC 1.A.1.2) subfamily. Kv3.1/KCNC1 sub-subfamily. -
Domain
The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
The tail may be important in modulation of channel activity and/or targeting of the channel to specific subcellular compartments. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 3746 Human
- Entrez Gene: 16502 Mouse
- Entrez Gene: 25327 Rat
- Omim: 176258 Human
- SwissProt: P48547 Human
- SwissProt: P15388 Mouse
- SwissProt: P25122 Rat
- Unigene: 552896 Human
see all -
Alternative names
- C230009H10Rik antibody
- FLJ41162 antibody
- FLJ42249 antibody
see all
Images
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PFA fixed, Triton X-100 permeabilized U-2 OS (Human bone osteosarcoma epithelial cell line) cells labeling KCNC1 using ab254906 at 4µg/ml (green) in ICC/IF.
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Paraffin-embedded human cerebellum tissue stained for KCNC1 using ab254906 at 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded human cerebral cortex tissue stained for KCNC1 using ab254906 at 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded human kidney tissue stained for KCNC1 using ab254906 at 1/500 dilution in immunohistochemical analysis. No positivity in cells in tubules as expected.
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Paraffin-embedded human small intestine tissue stained for KCNC1 using ab254906 at 1/500 dilution in immunohistochemical analysis. No positivity in glandular cells as expected.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab254906 has not yet been referenced specifically in any publications.