Key features and details
- Mouse monoclonal [S410-17] to KCNC2
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Product nameAnti-KCNC2 antibody [S410-17]
See all KCNC2 primary antibodies
DescriptionMouse monoclonal [S410-17] to KCNC2
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Fusion protein corresponding to Rat KCNC2 aa 474-613. (Cytoplasmic C-terminus)
Database link: P22462
- ICC/IF: SK-N-BE cells; WB: Rat brain tissue lysate; rat brain membrane lysate; mouse brain lysate; IHC-P: Human brain cortex tissue.
This product was previously labelled as Kv3.2
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.1% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab241999 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|WB||1/1000. Predicted molecular weight: 70 kDa.|
RelevancePotassium channels contribute to maintaining cell volume, membrane potential, neuronal excitability and the secretion of transmitters, salt and hormones. Two families of potassium channels have been identified. One family includes the inwardly rectifying potassium channels whereas, the other family includes: voltage sensing (KV); big conductance, calcium activated (BKca); and small conductance, calcium activated (SK) potassium channels. Kv3.2 functions as a delayed rectifier type K+ channel activated by large membrane depolarizations.
Cellular localizationCell Membrane
- KCNC2 antibody
- KSHIIIA antibody
- Kv 3.2 antibody
4% formaldehyde-fixed SK-N-BE (human neuroblastoma cell line) cells stained for KCNC2 (green, C) using ab241999 at 1/100 dilution for 1 hour at room temperature in ICC/IF. Secondary antibody is Goat Anti-Mouse ATTO 488 at 1/100 dilution (1 hr at RT). Actin is detected with Phalloidin Texas Red F-Actin stain (red, B). The nuclear counterstain is DAPI (blue, A). (D) is a composite.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain cortex tissue labeling KCNC2 with ab241999.
All lanes : Anti-KCNC2 antibody [S410-17] (ab241999) at 1/1000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain membrane lysate
Lane 3 : Rat brain tissue lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Mouse IgG: HRP at 1/200 dilution
Predicted band size: 70 kDa
Primary incubation for 16 hours at 4°C.
Secondary incubation for 1 hr at RT.
Blocked for 1 hr at RT.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab241999 has not yet been referenced specifically in any publications.