Anti-KCNC4 antibody [N72/16] (ab93605)

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Immunocytochemistry/ Immunofluorescence - Anti-KCNC4 antibody [N72/16] (ab93605)
  • Western blot - Anti-KCNC4 antibody [N72/16] (ab93605)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNC4 antibody [N72/16] (ab93605)

Key features and details

  • Mouse monoclonal [N72/16] to KCNC4
  • Suitable for: WB, ICC/IF, IHC-P
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-KCNC4 antibody [N72/16]

  • Description

    Mouse monoclonal [N72/16] to KCNC4

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide:

    GDEAGDDERELALQRLGP

    , corresponding to amino acids 175-192 of Rat KCNC4 (UniProt Q63734).
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: Rat brain membrane lysate. ICC/IF: HaCaT cells. IHC-P: Mouse back skin tissue.

  • General notes

    The clone number has been updated from S72-16 to N72/16, both clone numbers name the same antibody clone.

     

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab93605 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Predicted molecular weight: 68 kDa.
ICC/IF
Use a concentration of 1 - 10 µg/ml.
IHC-P
Use at an assay dependent concentration.
Notes
WB
1/1000. Predicted molecular weight: 68 kDa.
ICC/IF
Use a concentration of 1 - 10 µg/ml.
IHC-P
Use at an assay dependent concentration.

Target

  • Relevance

    KCNC4 (Potassium voltage gated channel subfamily C member 4) belongs to the delayed rectifier class of channel proteins and is an integral membrane protein that mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient.

  • Cellular localization

    Membrane; Multi-pass membrane protein.

  • Database links

    • Alternative names

      • HKSHIIIC antibody
      • KCNC 4 antibody
      • KSHIIIC antibody
      • KV3.4 antibody
      • MGC126818 antibody
      • Potassium voltage gated channel Shaw related subfamily member 4 antibody
      • Potassium voltage gated channel subfamily C member 4 antibody
      • Shaw related voltage gated potassium channel protein 4 antibody
      • Voltage gated potassium channel subunit KV3.4 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-KCNC4 antibody [N72/16] (ab93605)
      Immunocytochemistry/ Immunofluorescence - Anti-KCNC4 antibody [N72/16] (ab93605)

      100% methanol-fixed HaCaT (human keratinocyte cell line) cells stained for KCNC4 using ab93605 at 1/100 dilution in ICC/IF. Secondary antibody is FITC Goat Anti-Mouse.

       

    • Western blot - Anti-KCNC4 antibody [N72/16] (ab93605)
      Western blot - Anti-KCNC4 antibody [N72/16] (ab93605)
      Western blot detection of KCNC4 in Rat brain membrane lysates using ab93605 at 1:1000 dilution.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNC4 antibody [N72/16] (ab93605)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNC4 antibody [N72/16] (ab93605)

      Paraffin-embedded mouse back skin tissue stained for KCNC4 using ab93605 at 1/100 dilution in immunohistochemical analysis. Secondary antibody is FITC Goat Anti-Mouse.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab93605? Please let us know so that we can cite the reference in this datasheet.

    ab93605 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Question

    How do the five KCNC4 antibodies on the Abcam website differ from one another?

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    Answer

    Thank you for contacting us. As I mentioned in our conversation, and have confirmed, the immunogen sequences for these antibodies, aside from the immunogen of the mouse monoclonal ab93605 which is given on the datasheet, are not available to us as they are considered proprietary. The descriptions we do have and which you have seen are: ab66683 - Synthetic peptide derived from the N terminal domain of human KCNC4 ab79173 - Synthesized non phosphopeptide derived from Human KCNC4 around the phosphorylation site of serine 15 (R-K-SP-G-N) ab101780 - A synthetic peptide from the C-terminal region of Human KCNC4 ab101781 - A synthetic peptide from C-terminal region of Mouse KCNC4 We do know that the first two differ at least in their formulations (serum vs. purified). The second two, ab101780 and ab101781, differ in the species that they are raised against. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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