Anti-EAG1 antibody (ab101174)
Key features and details
- Rabbit polyclonal to EAG1
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
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- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-EAG1 antibody
See all EAG1 primary antibodies -
Description
Rabbit polyclonal to EAG1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Cow, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- This antibody gave a positive signal in the following tissue lysates: Rat Hippocampus; Rat Whole Brain; Mouse Hippocampus; Mouse Whole Brain.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab101174 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 108 kDa (predicted molecular weight: 108 kDa).
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 108 kDa (predicted molecular weight: 108 kDa). |
Target
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Function
Pore-forming (alpha) subunit of voltage-gated non-inactivating delayed rectifier potassium channel. Channel properties may be modulated by cAMP and subunit assembly. Mediates IK(NI) current in myoblasts. -
Tissue specificity
Highly expressed in brain and in myoblasts at the onset of fusion, but not in other tissues. Detected in HeLa (cervical carcinoma), SH-SY5Y (neuroblastoma) and MCF-7 (epithelial tumor) cells, but not in normal epithelial cells. -
Sequence similarities
Belongs to the potassium channel family. H (Eag) (TC 1.A.1.20) subfamily. Kv10.1/KCNH1 sub-subfamily.
Contains 1 cyclic nucleotide-binding domain.
Contains 1 PAC (PAS-associated C-terminal) domain.
Contains 1 PAS (PER-ARNT-SIM) domain. -
Domain
The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 281881 Cow
- Entrez Gene: 3756 Human
- Entrez Gene: 16510 Mouse
- Entrez Gene: 65198 Rat
- Omim: 603305 Human
- SwissProt: O18965 Cow
- SwissProt: O95259 Human
- SwissProt: Q60603 Mouse
see all -
Alternative names
- EAG antibody
- EAG channel 1 antibody
- EAG1 antibody
see all
Images
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All lanes : Anti-EAG1 antibody (ab101174) at 1 µg/ml
Lane 1 : Rat Hippocampus Tissue Lysate
Lane 2 : Brain (Rat) Tissue Lysate
Lane 3 : Mouse Hippocampus Tissue Lysate
Lane 4 : Brain (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 108 kDa
Observed band size: 108 kDa
Additional bands at: 55 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab101174 has been referenced in 1 publication.
- Li Z et al. Circ_0016347 Promotes Osteosarcoma Progression by Regulating miR-1225-3p/KCNH1 Axis. Cancer Biother Radiopharm N/A:N/A (2021). PubMed: 33764794