Overview

  • Product name
  • Description
    Rabbit polyclonal to KCNH2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human KCNH2 conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q12809

  • Positive control
    • HeLa cell lysate and Human heart tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab196301 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250 - 1/500. Predicted molecular weight: 127 kDa.
IHC-P 1/50 - 1/500.

Target

  • Function
    Pore-forming (alpha) subunit of voltage-gated inwardly rectifying potassium channel. Channel properties are modulated by cAMP and subunit assembly. Mediates the rapidly activating component of the delayed rectifying potassium current in heart (IKr). Isoform 3 has no channel activity by itself, but modulates channel characteristics when associated with isoform 1.
  • Tissue specificity
    Highly expressed in heart and brain.
  • Involvement in disease
    Defects in KCNH2 are the cause of long QT syndrome type 2 (LQT2) [MIM:613688]. Long QT syndromes are heart disorders characterized by a prolonged QT interval on the ECG and polymorphic ventricular arrhythmias. They cause syncope and sudden death in response to exercise or emotional stress. Deafness is often associated with LQT2.
    Defects in KCNH2 are the cause of short QT syndrome type 1 (SQT1) [MIM:609620]. Short QT syndromes are heart disorders characterized by idiopathic persistently and uniformly short QT interval on ECG in the absence of structural heart disease in affected individuals. They cause syncope and sudden death.
  • Sequence similarities
    Belongs to the potassium channel family. H (Eag) (TC 1.A.1.20) subfamily. Kv11.1/KCNH2 sub-subfamily.
    Contains 1 cyclic nucleotide-binding domain.
    Contains 1 PAC (PAS-associated C-terminal) domain.
    Contains 1 PAS (PER-ARNT-SIM) domain.
  • Domain
    The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
  • Post-translational
    modifications
    Phosphorylated on serine and threonine residues. Phosphorylation by PKA inhibits ion conduction.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • eag homolog antibody
    • Eag-related protein 1 antibody
    • ERG antibody
    • ERG-1 antibody
    • ERG1 antibody
    • Ether a go go related potassium channel protein antibody
    • Ether-a-go-go-related gene potassium channel 1 antibody
    • Ether-a-go-go-related protein 1 antibody
    • H ERG antibody
    • H-ERG antibody
    • HERG 1 antibody
    • HERG antibody
    • hERG-1 antibody
    • hERG1 antibody
    • KCNH2 antibody
    • KCNH2_HUMAN antibody
    • Kv11.1 antibody
    • LQT 2 antibody
    • LQT2 antibody
    • Potassium channel HERG antibody
    • Potassium voltage gated channel subfamily H (eag related) member 2 antibody
    • Potassium voltage-gated channel subfamily H member 2 antibody
    • SQT1 antibody
    • Voltage gated potassium channel, subfamily H, member 2 antibody
    • Voltage-gated potassium channel subunit Kv11.1 antibody
    see all

Images

  • Immunohistochemical staining of paraffin embedded, formalin fixed Human heart tissue labeling KCNH2 using ab196301 at 1/200 dilution.

  • Anti-KCNH2 antibody (ab196301) at 1/500 dilution + HeLa cell lysate

    Predicted band size: 127 kDa

References

This product has been referenced in:
  • Xu T  et al. Anticancer effect of miR-96 inhibitor in bladder cancer cell lines. Oncol Lett 15:3814-3819 (2018). Read more (PubMed: 29467898) »
  • Zeng W  et al. Silencing of hERG1 Gene Inhibits Proliferation and Invasion, and Induces Apoptosis in Human Osteosarcoma Cells by Targeting the NF-?B Pathway. J Cancer 7:746-57 (2016). IHC-P, WB ; Human . Read more (PubMed: 27076857) »

See all 2 Publications for this product

Customer reviews and Q&As

Abreviews
Application
Western blot
Sample
Pig Tissue lysate - whole (Left ventricle tissue)
Gel Running Conditions
Reduced Denaturing (Gel 12%)
Loading amount
30 µg
Specification
Left ventricle tissue
Blocking step
5% Milk-TBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

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Verified customer

Submitted Feb 28 2018

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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