Overview

  • Product name

    Anti-KCNIP4 antibody [EPR18314]
    See all KCNIP4 primary antibodies
  • Description

    Rabbit monoclonal [EPR18314] to KCNIP4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human KCNIP4 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: Q6PIL6

  • Positive control

    • WB: Human fetal brain lysate; Mouse and rat brain lysates; Rat and mouse cerebellum lysates; Human cerebellum and midbrain lysates. IP: Mouse brain whole cell lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab203831 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 29 kDa).
IP 1/50.

Target

  • Function

    Regulatory subunit of Kv4/D (Shal)-type voltage-gated rapidly inactivating A-type potassium channels. Probably modulates channels density, inactivation kinetics and rate of recovery from inactivation in a calcium-dependent and isoform-specific manner. In vitro, modulates KCND2/Kv4.2 and KCND3/Kv4.3 currents.
  • Tissue specificity

    Predominantly expressed in brain.
  • Sequence similarities

    Belongs to the recoverin family.
    Contains 4 EF-hand domains.
  • Domain

    The KIS (K-channel inactivation suppressor) domain is required for converting A-type Kv4 current to a slowly inactivating delayed rectifier potassium current.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • A type potassium channel modulatory protein 4 antibody
    • A-type potassium channel modulatory protein 4 antibody
    • CALP antibody
    • Calsenilin like protein antibody
    • Calsenilin-like protein antibody
    • HGNC:30083 antibody
    • KCHIP 4 antibody
    • KChIP4 antibody
    • KCIP4_HUMAN antibody
    • KCNIP 4 antibody
    • Kcnip4 antibody
    • Kv channel interacting protein 4 antibody
    • Kv channel-interacting protein 4 antibody
    • MGC44947 antibody
    • Potassium channel interacting protein 4 antibody
    • Potassium channel-interacting protein 4 antibody
    see all

Images

  • All lanes : Anti-KCNIP4 antibody [EPR18314] (ab203831) at 1/1000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Human fetal heart lysate
    Lane 3 : Human fetal kidney lysate
    Lane 4 : Human fetal spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 29 kDa
    Observed band size: 28 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 minute


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-KCNIP4 antibody [EPR18314] (ab203831) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse heart lysate
    Lane 3 : Mouse kidney lysate
    Lane 4 : Mouse spleen lysate
    Lane 5 : Rat brain lysate
    Lane 6 : Rat heart lysate
    Lane 7 : Rat kidney lysate
    Lane 8 : Rat spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 29 kDa
    Observed band size: 28 kDa why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-KCNIP4 antibody [EPR18314] (ab203831) at 1/1000 dilution

    Lane 1 : Rat cerebellum lysate
    Lane 2 : Mouse cerebellum lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 29 kDa
    Observed band size: 28 kDa why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-KCNIP4 antibody [EPR18314] (ab203831) at 1/10000 dilution + Human cerebellum lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 29 kDa
    Observed band size: 28 kDa why is the actual band size different from the predicted?


    Exposure time: 1 second


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-KCNIP4 antibody [EPR18314] (ab203831) at 1/10000 dilution + Human midbrain lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 29 kDa
    Observed band size: 28 kDa why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • KCNIP4 was immunoprecipitated from 1mg of Mouse brain whole cell lysate with ab203831 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab203831 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.

    Lane 1: Mouse brain whole cell lysate 10ug (Input). Lane 2: ab203831 IP in Mouse brain whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab203831 in Mouse brain whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds.

References

ab203831 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Dog Tissue lysate - whole (Cerebellum)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
Cerebellum
Blocking step
WesternBreeze blocking solution as blocking agent for 30 minute(s) · Concentration: 30% · Temperature: 21°C

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Verified customer

Submitted Mar 12 2019

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