Key features and details
- Rabbit Polyclonal to KCNJ14
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-KCNJ14 antibody
DescriptionRabbit Polyclonal to KCNJ14
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
- WB: RT4 and U-251 MG cell lysates. ICC/IF: HaCaT cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab221889 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 48 kDa.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
FunctionInward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. KCNJ14 gives rise to low-conductance channels with a low affinity to the channel blockers Barium and Cesium.
Tissue specificityExpressed preferentially in retina.
Sequence similaritiesBelongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ14 subfamily.
- Information by UniProt
- ATP-sensitive inward rectifier potassium channel 14 antibody
- Inward rectifier K(+) channel Kir2.4 antibody
- inwardly rectifying subfamily J member 14 antibody
PFA-fixed, Triton X-100 permeabilized HaCaT (human keratinocyte cell line) cells stained for KCNJ14 (green) using ab221889 at 4 µg/ml in ICC/IF.
All lanes : Anti-KCNJ14 antibody (ab221889) at 1/100 dilution
Lane 1 : RT4 (human urinary bladder cancer cell line) cell lysate
Lane 2 : U-251 MG (human brain glioma cell line) cell lysate
Predicted band size: 48 kDa
ab221889 has not yet been referenced specifically in any publications.