Overview

  • Product name

    Anti-KCNJ14 antibody
  • Description

    Rabbit Polyclonal to KCNJ14
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human KCNJ14 aa 407-436.
    Sequence:

    ETEEGNGVETEDGAASPRVLTPTLALTLPP


    Database link: Q9UNX9

  • Positive control

    • WB: RT4 and U-251 MG cell lysates. ICC/IF: HaCaT cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 40% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab221889 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/250. Predicted molecular weight: 48 kDa.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Fixation/Permeabilization: PFA/Triton X-100.

Target

  • Function

    Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. KCNJ14 gives rise to low-conductance channels with a low affinity to the channel blockers Barium and Cesium.
  • Tissue specificity

    Expressed preferentially in retina.
  • Sequence similarities

    Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ14 subfamily.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • ATP-sensitive inward rectifier potassium channel 14 antibody
    • Inward rectifier K(+) channel Kir2.4 antibody
    • inwardly rectifying subfamily J member 14 antibody
    • IRK-4 antibody
    • IRK4 antibody
    • KCJ14_HUMAN antibody
    • Kcnj14 antibody
    • KIR2.4 antibody
    • Potassium channel antibody
    • Potassium channel inwardly rectifying subfamily J member 14 antibody
    • Potassium inwardly rectifying channel, subfamily J member 14 antibody
    • Potassium voltage gated channel subfamily J member 14 antibody
    see all

Images

  • PFA-fixed, Triton X-100 permeabilized HaCaT (human keratinocyte cell line) cells stained for KCNJ14 (green) using ab221889 at 4 µg/ml in ICC/IF.

  • All lanes : Anti-KCNJ14 antibody (ab221889) at 1/100 dilution

    Lane 1 : RT4 (human urinary bladder cancer cell line) cell lysate
    Lane 2 : U-251 MG (human brain glioma cell line) cell lysate

    Predicted band size: 48 kDa

References

ab221889 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab221889.
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