Key features and details
- Rabbit polyclonal to KCNJ15
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-KCNJ15 antibody
See all KCNJ15 primary antibodies
DescriptionRabbit polyclonal to KCNJ15
Tested applicationsSuitable for: IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Guinea pig
- IHC-P: Human kidney, stomach, prostate and Fallopian tube tissue. ICC/IF: U-2 OS cells.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab224384 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
FunctionInward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium.
Sequence similaritiesBelongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ15 subfamily.
- Information by UniProt
- ATP sensitive inward rectifier potassium channel 15 antibody
- ATP-sensitive inward rectifier potassium channel 15 antibody
- Inward rectifier K(+) channel Kir1.3 antibody
Paraffin-embedded human Fallopian tube stained for KCNJ15 using ab224384 at 1/50 dilution in immunohistochemical analysis. Shows strong membranous positivity in glandular cells.
Paraffin-embedded human prostate stained for KCNJ15 using ab224384 at 1/50 dilution in immunohistochemical analysis. Shows strong membranous positivity in glandular cells.
Paraffin-embedded human stomach stained for KCNJ15 using ab224384 at 1/50 dilution in immunohistochemical analysis. Shows strong cytoplasmic and membranous positivity in glandular cells.
Paraffin-embedded human kidney stained for KCNJ15 using ab224384 at 1/50 dilution in immunohistochemical analysis. Shows strong cytoplasmic and membranous positivity in cells in tubules.
PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for KCNJ15 (green) using ab224384 at 4 µg/ml in ICC/IF.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab224384 has not yet been referenced specifically in any publications.