Key features and details
- Rabbit polyclonal to KCNJ5
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-KCNJ5 antibody
See all KCNJ5 primary antibodies
DescriptionRabbit polyclonal to KCNJ5
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Cow, Pig
- IHC: Human pancreas tissue. WB: KCNJ5 overexpression HEK-293T lysate.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab224396 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 48 kDa.|
FunctionThis potassium channel is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by external barium.
Tissue specificityIslets, exocrine pancreas and heart.
Involvement in diseaseDefects in KCNJ5 are the cause of long QT syndrome type 13 (LQT13) [MIM:613485]. It is a heart disorder characterized by a prolonged QT interval on the ECG and polymorphic ventricular arrhythmias. They cause syncope and sudden death in response to excercise or emotional stress, and can present with a sentinel event of sudden cardiac death in infancy.
Sequence similaritiesBelongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ5 subfamily.
- Information by UniProt
- Cardiac ATP sensitive potassium channel antibody
- Cardiac inward rectifier antibody
- CIR antibody
Paraffin-embedded human adrenal gland stained for KCNJ5 using ab224396 at 1/200 dilution in immunohistochemical analysis. Shows moderate to strong membranous positivity in glandular cells.
Paraffin-embedded human skeletal muscle stained for KCNJ5 using ab224396 at 1/200 dilution in immunohistochemical analysis. Shows no positivity in myocytes as expected .
Paraffin-embedded human skin stained for KCNJ5 using ab224396 at 1/200 dilution in immunohistochemical analysis. Shows no positivity in squamous epithelial cells as expected.
Paraffin-embedded human pacreas stained for KCNJ5 using ab224396 at 1/200 dilution in immunohistochemical analysis. Shows moderate to strong membranous positivity in exocrine glandular cells.
All lanes : Anti-KCNJ5 antibody (ab224396) at 1/100 dilution
Lane 1 : Vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) lysate (negative control)
Lane 2 : KCNJ5 overexpression HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) lysate
Predicted band size: 48 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab224396 has not yet been referenced specifically in any publications.