Key features and details
- Rabbit polyclonal to KCNK18/TRESK
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-KCNK18/TRESK antibody
See all KCNK18/TRESK primary antibodies
DescriptionRabbit polyclonal to KCNK18/TRESK
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
A 17 amino acid peptide from near the center of Human KCNK18/TRESK (NP_862823).
- Rat brain tissue IF: SKNSH cell line
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
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Storage instructionsShipped at 4°C. Store at 4°C (stable for up to 12 months).
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab106411 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 44 kDa.|
|IHC-P||Use a concentration of 5 µg/ml.|
FunctionOutward rectifying potassium channel. Produces rapidly activating outward rectifier K(+) currents. May function as background potassium channel that sets the resting membrane potential. Channel activity is directly activated by calcium signal. Activated by the G(q)-protein coupled receptor pathway. The calcium signal robustly activates the channel via calcineurin, whereas the anchoring of 14-3-3/YWHAH interferes with the return of the current to the resting state after activation. Inhibited also by arachidonic acid and other naturally occurring unsaturated free fatty acids. Channel activity is also enhanced by volatile anesthetics, such as isoflurane. Appears to be the primary target of hydroxy-alpha-sanshool, an ingredient of Schezuan pepper. May be involved in the somatosensory function with special respect to pain sensation.
Tissue specificityExpressed specifically in dorsal root ganglion and trigeminal ganglion neurons. Detected at low levels in spinal cord.
Involvement in diseaseDefects in KCNK18 are a cause of migraine with or without aura type 13 (MGR13) [MIM:613656]. A form of migraine trasmitted in an autosomal dominant pattern. Migraine is a disabling symptom complex of periodic headaches, usually temporal and unilateral. Headaches are often accompanied by irritability, nausea, vomiting and photobia, preceded by constriction of the cranial arteries. The two major subtypes are common migraine (migraine without aura) and classic migraine (migraine with aura). Classic migraine is characterized by recurrent attacks of reversible neurological symptoms (aura) that precede or accompany the headache. Aura may include a combination of sensory disturbances, such as blurred vision, hallucinations, vertigo, numbness and difficulty in concentrating and speaking. Note=Susceptibility to migraine has been shown to be conferred by a frameshift mutation that segregates with the disorder in a large multigenerational family. Migraine was associated with sensitivity to lights, sounds, and smells, as well as nausea and occasional vomiting. Triggers included fatigue, alcohol and bright lights. Mutations in KCNK18 are a rare cause of migraine.
Sequence similaritiesBelongs to the two pore domain potassium channel (TC 1.A.1.8) family.
modificationsPhosphorylation of Ser-252 is required for the binding of 14-3-3eta/YWHAH. Calcineurin-mediated dephosphorylation of Ser-264 enhances channel activity.
Cellular localizationCell membrane.
- Information by UniProt
- K2p18.1 antibody
- KCNK18 antibody
- KCNKI_HUMAN antibody
ab106411, at 5 µg/ml, staining KCNK18/TRESK in paraffin embedded Rat brain tissue.
Lane 1 : Anti-KCNK18/TRESK antibody (ab106411) at 1 µg/ml
Lane 2 : Anti-KCNK18/TRESK antibody (ab106411) at 2 µg/ml
All lanes : Rat brain tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 44 kDa
ICC/IF image of ab106411 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab106411, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunofluorescence of TRESK in Rat Brain cells using ab106411 at 20 ug/ml.
ab106411 has not yet been referenced specifically in any publications.