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    kcnk18tresk-antibody-ab106411.pdf

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Neuroscience Neurotransmission Receptors / Channels Potassium Channels
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Anti-KCNK18/TRESK antibody (ab106411)

  • Datasheet
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNK18/TRESK antibody (ab106411)
  • Western blot - Anti-KCNK18/TRESK antibody (ab106411)
  • Immunocytochemistry/ Immunofluorescence - Anti-KCNK18/TRESK antibody (ab106411)
  • Immunocytochemistry/ Immunofluorescence - Anti-KCNK18/TRESK antibody (ab106411)

Key features and details

  • Rabbit polyclonal to KCNK18/TRESK
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human KCNK18/TRESK protein (ab132696)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-KCNK18/TRESK antibody
    See all KCNK18/TRESK primary antibodies
  • Description

    Rabbit polyclonal to KCNK18/TRESK
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    A 17 amino acid peptide from near the center of Human KCNK18/TRESK (NP_862823).

  • Positive control

    • Rat brain tissue IF: SKNSH cell line
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at 4°C (stable for up to 12 months).
  • Storage buffer

    pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • Potassium Channels
    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • GPCR
    • More GPCR
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Rat brain normal tissue lysate - membrane extract (ab29473)
  • Recombinant Protein

    • Recombinant Human KCNK18/TRESK protein (ab132696)

Applications

Our Abpromise guarantee covers the use of ab106411 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 44 kDa.
IHC-P Use a concentration of 5 µg/ml.

Target

  • Function

    Outward rectifying potassium channel. Produces rapidly activating outward rectifier K(+) currents. May function as background potassium channel that sets the resting membrane potential. Channel activity is directly activated by calcium signal. Activated by the G(q)-protein coupled receptor pathway. The calcium signal robustly activates the channel via calcineurin, whereas the anchoring of 14-3-3/YWHAH interferes with the return of the current to the resting state after activation. Inhibited also by arachidonic acid and other naturally occurring unsaturated free fatty acids. Channel activity is also enhanced by volatile anesthetics, such as isoflurane. Appears to be the primary target of hydroxy-alpha-sanshool, an ingredient of Schezuan pepper. May be involved in the somatosensory function with special respect to pain sensation.
  • Tissue specificity

    Expressed specifically in dorsal root ganglion and trigeminal ganglion neurons. Detected at low levels in spinal cord.
  • Involvement in disease

    Defects in KCNK18 are a cause of migraine with or without aura type 13 (MGR13) [MIM:613656]. A form of migraine trasmitted in an autosomal dominant pattern. Migraine is a disabling symptom complex of periodic headaches, usually temporal and unilateral. Headaches are often accompanied by irritability, nausea, vomiting and photobia, preceded by constriction of the cranial arteries. The two major subtypes are common migraine (migraine without aura) and classic migraine (migraine with aura). Classic migraine is characterized by recurrent attacks of reversible neurological symptoms (aura) that precede or accompany the headache. Aura may include a combination of sensory disturbances, such as blurred vision, hallucinations, vertigo, numbness and difficulty in concentrating and speaking. Note=Susceptibility to migraine has been shown to be conferred by a frameshift mutation that segregates with the disorder in a large multigenerational family. Migraine was associated with sensitivity to lights, sounds, and smells, as well as nausea and occasional vomiting. Triggers included fatigue, alcohol and bright lights. Mutations in KCNK18 are a rare cause of migraine.
  • Sequence similarities

    Belongs to the two pore domain potassium channel (TC 1.A.1.8) family.
  • Post-translational
    modifications

    Phosphorylation of Ser-252 is required for the binding of 14-3-3eta/YWHAH. Calcineurin-mediated dephosphorylation of Ser-264 enhances channel activity.
    N-glycosylated.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession Q7Z418 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 338567 Human
    • Entrez Gene: 332396 Mouse
    • Entrez Gene: 445371 Rat
    • Omim: 613655 Human
    • SwissProt: Q7Z418 Human
    • SwissProt: Q6VV64 Mouse
    • SwissProt: Q6Q1P3 Rat
    • Unigene: 449650 Human
    • Unigene: 329947 Mouse
    • Unigene: 124394 Rat
    see all
  • Alternative names

    • K2p18.1 antibody
    • KCNK18 antibody
    • KCNKI_HUMAN antibody
    • MGR13 antibody
    • OTTHUMP00000020575 antibody
    • Potassium channel subfamily K member 18 antibody
    • TRESK 2 antibody
    • TRESK antibody
    • TRESK2 antibody
    • TRIK antibody
    • TWIK related individual K+ channel antibody
    • TWIK related individual potassium channel antibody
    • TWIK related spinal cord K+ channel antibody
    • TWIK related spinal cord potassium channel antibody
    • TWIK-related individual potassium channel antibody
    • TWIK-related spinal cord potassium channel antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNK18/TRESK antibody (ab106411)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNK18/TRESK antibody (ab106411)
    ab106411, at 5 µg/ml, staining KCNK18/TRESK in paraffin embedded Rat brain tissue.
  • Western blot - Anti-KCNK18/TRESK antibody (ab106411)
    Western blot - Anti-KCNK18/TRESK antibody (ab106411)
    Lane 1 : Anti-KCNK18/TRESK antibody (ab106411) at 1 µg/ml
    Lane 2 : Anti-KCNK18/TRESK antibody (ab106411) at 2 µg/ml

    All lanes : Rat brain tissue lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 44 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-KCNK18/TRESK antibody (ab106411)
    Immunocytochemistry/ Immunofluorescence - Anti-KCNK18/TRESK antibody (ab106411)
    ICC/IF image of ab106411 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab106411, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • Immunocytochemistry/ Immunofluorescence - Anti-KCNK18/TRESK antibody (ab106411)
    Immunocytochemistry/ Immunofluorescence - Anti-KCNK18/TRESK antibody (ab106411)
    Immunofluorescence of TRESK in Rat Brain cells using ab106411 at 20 ug/ml.

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab106411? Please let us know so that we can cite the reference in this datasheet.

    ab106411 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    Regarding Anti-KCNK18/TRESK antibody (ab106411):
    The datasheet states: At least four isoforms of KCNK18/TRESK are known to exist; ab106411 will recognize all four.
    Could you provide the references for the 4 isoforms?

    Read More

    Abcam community

    Verified customer

    Asked on Jul 27 2012

    Answer

    Thank you for contacting Abcam regarding ab106411.


    It appears that this text was added to the datasheet in error. This has now been removed.


    I apologize for any confusion this may have caused. Please do not hesitate to contact me if you have any additional questions.

    Read More

    Abcam Scientific Support

    Answered on Jul 27 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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