Key features and details
- Rabbit polyclonal to KCNK1/TWIK1
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-KCNK1/TWIK1 antibody
See all KCNK1/TWIK1 primary antibodies
DescriptionRabbit polyclonal to KCNK1/TWIK1
Tested applicationsSuitable for: IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Rabbit, Orangutan
- IHC: Human cerebellum tissue. ICC/IF: HeLa cells.
This product was previously labelled as KCNK1
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab224381 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
FunctionWeakly inward rectifying potassium channel.
Tissue specificityWidely expressed with high levels in heart and brain and lower levels in placenta, lung, liver and kidney.
Sequence similaritiesBelongs to the two pore domain potassium channel (TC 1.A.1.8) family.
- Information by UniProt
- DPK antibody
- HOHO antibody
- HOHO1 antibody
Paraffin-embedded human cerebellum tissue stained for KCNK1/TWIK1 using ab224381 at 1/200 dilution in immunohistochemical analysis.
PFA-fixed, Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for KCNK1/TWIK1 (green) using ab224381 at 4 µg/ml in ICC/IF.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab224381 has not yet been referenced specifically in any publications.