Overview

  • Product name

    Anti-KCNMB2 antibody [N53/32]
    See all KCNMB2 primary antibodies
  • Description

    Mouse monoclonal [N53/32] to KCNMB2
  • Host species

    Mouse
  • Specificity

    No cross-reactivity against BKBeta1, BKBeta3 or BKBeta4. Not yet validated for use in mammalian brain.
  • Tested applications

    Suitable for: WB, IHC-P, IHC-Fr, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Fusion protein corresponding to amino acids 1 - 41 (MFIWTSGRTS SSYRQDEKRN IYQKIRDHDL LDKRKTVTAL K; N-terminus) and 218 - 235 (KLTQYLSLLC ERIQRINR; C-terminus) of Mouse KCNMB2 (UniProt ID: Q9CZM9).

  • Positive control

    • WB: Human cell line mix lysates. COS-1 cells transiently transfected with KCNMB2 lysate.
  • General notes

    The clone number has been updated from S53-32 to N53/32, both clone numbers name the same antibody clone.

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.09% Sodium azide
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    N53/32
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab94589 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 27 kDa.
IHC-P 1/1000.
IHC-Fr 1/1000.
ICC/IF 1/100.

Target

  • Function

    Regulatory subunit of the calcium activated potassium KCNMA1 (maxiK) channel. Modulates the calcium sensitivity and gating kinetics of KCNMA1, thereby contributing to KCNMA1 channel diversity. Acts as a negative regulator that confers rapid and complete inactivation of KCNMA1 channel complex. May participate in KCNMA1 inactivation in chromaffin cells of the adrenal gland or in hippocampal CA1 neurons.
  • Tissue specificity

    Expressed in kidney, heart and brain. Highly expressed in ovary. Expressed at low level in other tissues.
  • Sequence similarities

    Belongs to the KCNMB (TC 8.A.14.1) family. KCNMB2 subfamily.
  • Domain

    The ball and chain domain mediates the inactivation of KCNMA1. It occludes the conduction pathway of KCNMA1 channels, and comprises the pore-blocking ball domain (residues 1-17) and the chain domain (residues 20-45) linking it to the transmembrane segment. The ball domain is made up of a flexible N-terminus anchored at a well ordered loop-helix motif. The chain domain consists of a 4-turn helix with an unfolded linker at its C-terminus.
  • Post-translational
    modifications

    N-glycosylated.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2700049B16Rik antibody
    • 3110031N04Rik antibody
    • BK channel subunit beta 2 antibody
    • BK channel subunit beta-2 antibody
    • BKbeta2 antibody
    • Calcium activated potassium channel subfamily M subunit beta 2 antibody
    • Calcium activated potassium channel subunit beta 2 antibody
    • Calcium-activated potassium channel antibody
    • Calcium-activated potassium channel subunit beta-2 antibody
    • Charybdotoxin receptor subunit beta 2 antibody
    • Charybdotoxin receptor subunit beta-2 antibody
    • Hbeta2 antibody
    • Hbeta3 antibody
    • K(VCA)beta-2 antibody
    • K(VCA)beta2 antibody
    • KCMB2_HUMAN antibody
    • KCNMB 2 antibody
    • Kcnmb2 antibody
    • Large conductance Ca2+ activated K+ channel beta2 subunit antibody
    • Large conductance calcium activated potassium channel beta 2 subunit antibody
    • Maxi K channel subunit beta 2 antibody
    • Maxi K channel subunit beta-2 antibody
    • MaxiK channel beta 2 subunit antibody
    • MGC22431 antibody
    • MGC57945 antibody
    • Potassium large conductance calcium activated channel subfamily M beta member 2 antibody
    • Slo-beta-2 antibody
    • Slobeta2 antibody
    • subfamily M subunit beta-2 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of mouse backskin staining KCNMB2 with ab94589 at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.

  • Anti-KCNMB2 antibody [N53/32] (ab94589) at 1/1000 dilution + Human cell line mix lysates at 15 µg

    Secondary
    Secondary Antibody: Sheep Anti-Mouse IgG: HRP

    Predicted band size: 27 kDa



    Block: 1% BSA for 30 minutes at RT.

    Primary antibody incubated for 2 hours at RT.

    Secondary antibody incubated for 1 hour at RT.

References

ab94589 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab94589.
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