Key features and details
- Mouse monoclonal [N53/32] to KCNMB2
- Suitable for: WB, IHC-P, IHC-Fr, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-KCNMB2 antibody [N53/32]
DescriptionMouse monoclonal [N53/32] to KCNMB2
SpecificityNo cross-reactivity against BKBeta1, BKBeta3 or BKBeta4. Not yet validated for use in mammalian brain.
Tested applicationsSuitable for: WB, IHC-P, IHC-Fr, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Fusion protein corresponding to amino acids 1 - 41 (MFIWTSGRTS SSYRQDEKRN IYQKIRDHDL LDKRKTVTAL K; N-terminus) and 218 - 235 (KLTQYLSLLC ERIQRINR; C-terminus) of Mouse KCNMB2 (UniProt ID: Q9CZM9).
- WB: Human cell line mix lysates. COS-1 cells transiently transfected with KCNMB2 lysate.
The clone number has been updated from S53-32 to N53/32, both clone numbers name the same antibody clone.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab94589 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 27 kDa.|
FunctionRegulatory subunit of the calcium activated potassium KCNMA1 (maxiK) channel. Modulates the calcium sensitivity and gating kinetics of KCNMA1, thereby contributing to KCNMA1 channel diversity. Acts as a negative regulator that confers rapid and complete inactivation of KCNMA1 channel complex. May participate in KCNMA1 inactivation in chromaffin cells of the adrenal gland or in hippocampal CA1 neurons.
Tissue specificityExpressed in kidney, heart and brain. Highly expressed in ovary. Expressed at low level in other tissues.
Sequence similaritiesBelongs to the KCNMB (TC 8.A.14.1) family. KCNMB2 subfamily.
DomainThe ball and chain domain mediates the inactivation of KCNMA1. It occludes the conduction pathway of KCNMA1 channels, and comprises the pore-blocking ball domain (residues 1-17) and the chain domain (residues 20-45) linking it to the transmembrane segment. The ball domain is made up of a flexible N-terminus anchored at a well ordered loop-helix motif. The chain domain consists of a 4-turn helix with an unfolded linker at its C-terminus.
- Information by UniProt
- 2700049B16Rik antibody
- 3110031N04Rik antibody
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of mouse backskin staining KCNMB2 with ab94589 at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.
Anti-KCNMB2 antibody [N53/32] (ab94589) at 1/1000 dilution + Human cell line mix lysates at 15 µg
Secondary Antibody: Sheep Anti-Mouse IgG: HRP
Predicted band size: 27 kDa
Block: 1% BSA for 30 minutes at RT.
Primary antibody incubated for 2 hours at RT.
Secondary antibody incubated for 1 hour at RT.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab94589 has been referenced in 1 publication.
- Schlüter T et al. miR-96 is required for normal development of the auditory hindbrain. Hum Mol Genet 27:860-874 (2018). PubMed: 29325119