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  1. Link

    kcnmb3-antibody-epr9543b-ab137041.pdf

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Neuroscience Neurotransmission Receptors / Channels Potassium Channels
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RecombinantRabMAb

Recombinant Anti-KCNMB3 antibody [EPR9543(B)] (ab137041)

  • Datasheet
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Western blot - Anti-KCNMB3 antibody [EPR9543(B)] (ab137041)
  • Flow Cytometry (Intracellular) - Anti-KCNMB3 antibody [EPR9543(B)] (ab137041)
  • Anti-KCNMB3 antibody [EPR9543(B)] (ab137041)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9543(B)] to KCNMB3
  • Suitable for: Flow Cyt (Intra), WB
  • Reacts with: Mouse, Rat, Human

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Carrier Free

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Overview

  • Product name

    Anti-KCNMB3 antibody [EPR9543(B)]
    See all KCNMB3 primary antibodies
  • Description

    Rabbit monoclonal [EPR9543(B)] to KCNMB3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), WBmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • BxPC3, HepG2, C6, RAW 264.7, PC12 and NIH 3T3 cell lysates; HeLa cells
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR9543(B)
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • Potassium Channels
    • Signal Transduction
    • Metabolism
    • Plasma Membrane
    • Channels

Associated products

  • Alternative Versions

    • Anti-KCNMB3 antibody [EPR9543(B)] - BSA and Azide free (ab248782)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
    • NIH/3T3 whole cell lysate (ab7179)
    • RAW 264.7 whole cell lysate (ab7187)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab137041 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/1000 - 1/10000. Detects a band of approximately 29 - 32 kDa (predicted molecular weight: 32 kDa).
Notes
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/1000 - 1/10000. Detects a band of approximately 29 - 32 kDa (predicted molecular weight: 32 kDa).
Application notes
Is unsuitable for ICC/IF,IHC-P or IP.

Target

  • Function

    Regulatory subunit of the calcium activated potassium KCNMA1 (maxiK) channel. Modulates the calcium sensitivity and gating kinetics of KCNMA1, thereby contributing to KCNMA1 channel diversity. Alters the functional properties of the current expressed by the KCNMA1 channel. Isoform 2, isoform 3 and isoform 4 partially inactivate the current of KCNBMA. Isoform 4 induces a fast and incomplete inactivation of KCNMA1 channel that is detectable only at large depolarizations. In contrast, isoform 1 does not induce detectable inactivation of KCNMA1. Two or more subunits of KCNMB3 are required to block the KCNMA1 tetramer.
  • Tissue specificity

    Isoform 1, isoform 3 and isoform 4 are widely expressed. Isoform 2 is expressed placenta, pancreas, kidney and heart. Isoform 1 and isoform 3 are highly expressed in pancreas and testis.
  • Sequence similarities

    Belongs to the KCNMB (TC 8.A.14.1) family. KCNMB3 subfamily.
  • Domain

    Isoform 4 cytoplasmic N-terminal domain participates in the partial inactivation of KCNMA1, possibly by binding to a receptor site.
    The extracellular domain forms gates to block ion permeation, providing a mechanism by which current can be rapidly diminished upon cellular repolarization.
  • Post-translational
    modifications

    N-glycosylated.
    The extracellular domain contains disulfide bond essential for the gating mechanism.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession Q9NPA1 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 27094 Human
    • Entrez Gene: 435726 Mouse
    • Entrez Gene: 310303 Rat
    • Omim: 605222 Human
    • SwissProt: Q9NPA1 Human
    • SwissProt: A7VL23 Rat
    • Unigene: 591285 Human
    • Unigene: 218452 Rat
    • Alternative names

      • BK channel subunit beta 3 antibody
      • BK channel subunit beta-3 antibody
      • BKbeta 3 antibody
      • BKbeta3 antibody
      • Calcium activated potassium channel beta 3 subunit antibody
      • Calcium activated potassium channel subfamily M subunit beta 3 antibody
      • Calcium activated potassium channel subunit beta 3 antibody
      • Calcium-activated potassium channel antibody
      • Calcium-activated potassium channel subunit beta-3 antibody
      • Charybdotoxin receptor subunit beta 3 antibody
      • Charybdotoxin receptor subunit beta-3 antibody
      • EG435726 antibody
      • Gm5707 antibody
      • Hbeta 3 antibody
      • Hbeta3 antibody
      • K(VCA)beta 3 antibody
      • K(VCA)beta-3 antibody
      • KCMB3_HUMAN antibody
      • KCNMB 2 antibody
      • KCNMB 3 antibody
      • KCNMB2 antibody
      • KCNMB3 antibody
      • KCNMBL antibody
      • Large conductance voltage and Ca2+ activated potassium channel Maxi K beta 3 subunit antibody
      • Maxi K channel subunit beta 3 antibody
      • Maxi K channel subunit beta-3 antibody
      • Potassium channel, calcium-activated large conductance, subfamily M, beta member 3 antibody
      • Potassium large conductance calcium activated channel beta 3 subunit antibody
      • Potassium large conductance calcium activated channel subfamily M beta member 3 antibody
      • Slo beta 3 antibody
      • Slo-beta-3 antibody
      • subfamily M subunit beta-3 antibody
      see all

    Images

    • Western blot - Anti-KCNMB3 antibody [EPR9543(B)] (ab137041)
      Western blot - Anti-KCNMB3 antibody [EPR9543(B)] (ab137041)
      All lanes : Anti-KCNMB3 antibody [EPR9543(B)] (ab137041) at 1/1000 dilution

      Lane 1 : BxPC3 cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : C6 cell lysate
      Lane 5 : RAW 264.7 cell lysate
      Lane 6 : PC12 cell lysate
      Lane 7 : NIH 3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP conjugated Goat anti Rabbit IgG at 1/2000 dilution

      Predicted band size: 32 kDa

    • Flow Cytometry (Intracellular) - Anti-KCNMB3 antibody [EPR9543(B)] (ab137041)
      Flow Cytometry (Intracellular) - Anti-KCNMB3 antibody [EPR9543(B)] (ab137041)

      Intracellular Flow Cytometry analysis of permeabilized Hela cells labelling KCNMB3 with ab137041 at 1/10 dilution (Red) or a Rabbit IgG (negative control) (Green).

       

    • Anti-KCNMB3 antibody [EPR9543(B)] (ab137041)
      Anti-KCNMB3 antibody [EPR9543(B)] (ab137041)

    Protocols

    • Western blot protocols
    • Flow cytometry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (2)

    Publishing research using ab137041? Please let us know so that we can cite the reference in this datasheet.

    ab137041 has been referenced in 2 publications.

    • Zaidman NA  et al. Large-conductance Ca(2+) -activated K(+) channel activation by apical P2Y receptor agonists requires hydrocortisone in differentiated airway epithelium. J Physiol 595:4631-4645 (2017). PubMed: 28481415
    • Petho Z  et al. Different expression of ß subunits of the KCa1.1 channel by invasive and non-invasive human fibroblast-like synoviocytes. Arthritis Res Ther 18:103 (2016). PubMed: 27165430

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