Product nameAnti-KCNN2 / SK2 antibody
See all KCNN2 / SK2 primary antibodies
DescriptionRabbit polyclonal to KCNN2 / SK2
SpecificityThis antibody recognizes KCNN2.
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow, Zebrafish
- WB: HepG2 cell lysate.
Protein previously known as KCNN2.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab48817 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1.25 µg/ml. Predicted molecular weight: 64 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|ELISA||Use at an assay dependent concentration.
ELISA titre using peptide based assay was 1:62500.
FunctionForms a voltage-independent potassium channel activated by intracellular calcium. Activation is followed by membrane hyperpolarization. Thought to regulate neuronal excitability by contributing to the slow component of synaptic afterhyperpolarization. The channel is blocked by apamin.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the potassium channel KCNN family. KCa2.2/KCNN2 subfamily.
- Information by UniProt
- Apamin sensitive small conductance Ca2+ activated potassium antibody
- Apamin sensitive small conductance Ca2+ activated potassium channel antibody
- hSK2 antibody
Anti-KCNN2 / SK2 antibody (ab48817) at 1.25 µg/ml + HepG2 lysate at 10 µg with 5% skimmed milk in PBS.
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 64 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Gel concentration 12%
This product has been referenced in:
- Kushwah N et al. Hypobaric Hypoxia-Induced Learning and Memory Impairment: Elucidating the Role of Small Conductance Ca2+-Activated K+ Channels. Neuroscience 388:418-429 (2018). Read more (PubMed: 30048783) »
- Willis M et al. Small-conductance calcium-activated potassium type 2 channels (SK2, KCa2.2) in human brain. Brain Struct Funct 222:973-979 (2017). WB ; Human . Read more (PubMed: 27357310) »