Overview

  • Product name

  • Description

    Goat polyclonal to KCNN2/SK2
  • Host species

    Goat
  • Specificity

    Recognizes both Human isoforms (NP_067627.2 and NP_740721.1)
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Guinea pig, Cow, Dog, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human KCNN2/SK2 (internal sequence) (Cysteine residue). Corresponding to amino acids 546 - 557 of human KCNN2/SK2 (NP_067627.2) and 197 - 209 of human KCNN2/SK2 isoform b (NP_740721.1).
    Sequence:

    (c)ESYDKHVTYNAER


    (Peptide available as ab186680)

  • Positive control

    • WB: Human liver lysate. IHC-P: Human thyroid tissue.
  • General notes

    Protein previously known as KCNN2.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab99457 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 - 2 µg/ml.
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 70, 23 kDa (predicted molecular weight: 64, 26 kDa).Can be blocked with KCNN2/SK2 peptide (ab186680).

Target

Images

  • Anti-KCNN2/SK2 antibody (ab99457) at 0.3 µg/ml + Human liver lysate, in RIPA buffer at 35 µg

    Developed using the ECL technique.

    Predicted band size: 64, 26 kDa
    Observed band size: 23,70 kDa
    why is the actual band size different from the predicted?



    Primary incubation was 1 hour.
  • ab99457 at 3.8 µg/ml staining KCNN2/SK2 in human thyroid by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6 was performed, antibody was revealed by AP-staining.

References

ab99457 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (spinal cord)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate, pH6
Permeabilization
Yes - 0.1% Tween-20
Specification
spinal cord
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde

Mrs. Teresa Garrett

Verified customer

Submitted Oct 28 2016

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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