Key features and details
- Rabbit polyclonal to KCNN4
- Suitable for: IHC-P, WB
- Reacts with: Rat
- Isotype: IgG
Product nameAnti-KCNN4 antibody
See all KCNN4 primary antibodies
DescriptionRabbit polyclonal to KCNN4
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Rat
Predicted to work with: Mouse, Human
- Rat brain tissue; rat brain and rat heart lysates.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1% BSA, 50% Glycerol
Aqueous buffered solution
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab215990 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/100 - 1/1000. Predicted molecular weight: 48 kDa.|
FunctionForms a voltage-independent potassium channel that is activated by intracellular calcium. Activation is followed by membrane hyperpolarization which promotes calcium influx. Required for maximal calcium influx and proliferation during the reactivation of naive T cells. The channel is blocked by clotrimazole and charybdotoxin but is insensitive to apamin.
Tissue specificityWidely expressed in non-excitable tissues.
Sequence similaritiesBelongs to the potassium channel KCNN family. KCa3.1/KCNN4 subfamily.
modificationsPhosphorylation at His-358 by NDKB activates the channel, and conversely it's dephosphorylation by PHPT1 inhibits the channel.
- Information by UniProt
- DHS2 antibody
- hIKCa1 antibody
- hKCa4 antibody
All lanes : Anti-KCNN4 antibody (ab215990) at 1/200 dilution
Lane 1 : rat brain lysate
Lane 2 : rat heart lysate
All lanes : HRP-conjugated secondary antibody at 1/3000 dilution
Predicted band size: 48 kDa
Observed band size: 45, 50 kDa why is the actual band size different from the predicted?
Immunohistochemical analysis of formalin-fixed and paraffin-embedded rat brain tissue labeling KCNN4 with ab215990 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
ab215990 has been referenced in 1 publication.
- Yang M et al. Adipose-derived stem cells overexpressing SK4 calcium-activated potassium channel generate biological pacemakers. Int J Mol Med 44:2103-2112 (2019). PubMed: 31638180