Overview

  • Product name

  • Description

    Rabbit polyclonal to KCNN4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Rat
    Predicted to work with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human KCNN4 aa 340-375 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Sequence:

    H QRKLLAAINA FRQVRLKHRK LREQVNSMVD ISKMH


    Database link: O15554

  • Positive control

    • Rat brain tissue; rat brain and rat heart lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab215990 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/1000. Predicted molecular weight: 48 kDa.

Target

  • Function

    Forms a voltage-independent potassium channel that is activated by intracellular calcium. Activation is followed by membrane hyperpolarization which promotes calcium influx. Required for maximal calcium influx and proliferation during the reactivation of naive T cells. The channel is blocked by clotrimazole and charybdotoxin but is insensitive to apamin.
  • Tissue specificity

    Widely expressed in non-excitable tissues.
  • Sequence similarities

    Belongs to the potassium channel KCNN family. KCa3.1/KCNN4 subfamily.
  • Post-translational
    modifications

    Phosphorylation at His-358 by NDKB activates the channel, and conversely it's dephosphorylation by PHPT1 inhibits the channel.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • DHS2 antibody
    • hIKCa1 antibody
    • hKCa4 antibody
    • hSK4 antibody
    • IK antibody
    • IK1 antibody
    • IKCa1 antibody
    • Intermediate conductance calcium activated potassium channel protein 4 antibody
    • Intermediate conductance calcium-activated potassium channel protein 4 antibody
    • KCa3.1 antibody
    • KCa4 antibody
    • KCNN 4 antibody
    • Kcnn4 antibody
    • KCNN4_HUMAN antibody
    • Potassium calcium activated channel subfamily N member 4 antibody
    • Potassium intermediate/small conductance calcium activated channel, subfamily N, member 4 antibody
    • Putative Gardos channel antibody
    • SK4 antibody
    • SKCa 4 antibody
    • SKCa4 antibody
    see all

Images

  • All lanes : Anti-KCNN4 antibody (ab215990) at 1/200 dilution

    Lane 1 : rat brain lysate
    Lane 2 : rat heart lysate

    Secondary
    All lanes : HRP-conjugated secondary antibody at 1/3000 dilution

    Predicted band size: 48 kDa
    Observed band size: 45, 50 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemical analysis of formalin-fixed and paraffin-embedded rat brain tissue labeling KCNN4 with ab215990 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

References

ab215990 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab215990.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up