Overview

  • Product name

  • Description

    Rabbit polyclonal to KCNN4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human, Pig
  • Immunogen

    Synthetic peptide corresponding to Human KCNN4 aa 400 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab87266)

  • Positive control

    • This antibody gave a positive signal in the following human tissue lysates: Skin Placenta

Properties

Applications

Our Abpromise guarantee covers the use of ab75956 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa).

Target

  • Function

    Forms a voltage-independent potassium channel that is activated by intracellular calcium. Activation is followed by membrane hyperpolarization which promotes calcium influx. Required for maximal calcium influx and proliferation during the reactivation of naive T cells. The channel is blocked by clotrimazole and charybdotoxin but is insensitive to apamin.
  • Tissue specificity

    Widely expressed in non-excitable tissues.
  • Sequence similarities

    Belongs to the potassium channel KCNN family. KCa3.1/KCNN4 subfamily.
  • Post-translational
    modifications

    Phosphorylation at His-358 by NDKB activates the channel, and conversely it's dephosphorylation by PHPT1 inhibits the channel.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • DHS2 antibody
    • hIKCa1 antibody
    • hKCa4 antibody
    • hSK4 antibody
    • IK antibody
    • IK1 antibody
    • IKCa1 antibody
    • Intermediate conductance calcium activated potassium channel protein 4 antibody
    • Intermediate conductance calcium-activated potassium channel protein 4 antibody
    • KCa3.1 antibody
    • KCa4 antibody
    • KCNN 4 antibody
    • Kcnn4 antibody
    • KCNN4_HUMAN antibody
    • Potassium calcium activated channel subfamily N member 4 antibody
    • Potassium intermediate/small conductance calcium activated channel, subfamily N, member 4 antibody
    • Putative Gardos channel antibody
    • SK4 antibody
    • SKCa 4 antibody
    • SKCa4 antibody
    see all

Images

  • All lanes : Anti-KCNN4 antibody (ab75956) at 1 µg/ml

    Lane 1 : Human skin tissue lysate - total protein (ab30166)
    Lane 2 : Human placenta tissue lysate - total protein (ab29745)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 47 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?



    The 50 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to KCNN4.

References

This product has been referenced in:

  • Morales P  et al. Contribution of the KCa3.1 channel-calmodulin interactions to the regulation of the KCa3.1 gating process. J Gen Physiol 142:37-60 (2013). WB . Read more (PubMed: 23797421) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Question
Answer

Thank you for your patience. Here is the homology between human and mouse for the following antibodies (based on the alignment of their immunogen sequences) ab55452: homology = 94% - crossreactivity very likely, but not tested ab57662: homology = 96% - crossreactivity very likely, but not tested ab70477: homology = 36.4% - crossreactivity not very likely, but not tested ab75956: homology = 63% - crossreactivity not likely, but not tested ab119295: homology = 98% - crossreactivity very likely, but not tested Since non-crossreactivity or crossreactivity with mouse has not been tested, we would not be able to guarantee that the the antibodies will not work / work with mouse. For ab106987, I could not find the mouse sequence and am checking with the lab about that. Should you have access to such sequence for C1orf186 please let me know as well. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More
Application
Western blot
Sample
Pig Tissue lysate - whole (Right Coronary Artery & Lung)
Loading amount
20 µg
Specification
Right Coronary Artery & Lung
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (4-20% Tris Ready Gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

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Verified customer

Submitted Aug 02 2010

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