Key features and details
- Rabbit polyclonal to KCNN4
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-KCNN4 antibody
See all KCNN4 primary antibodies
DescriptionRabbit polyclonal to KCNN4
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Pig
Synthetic peptide corresponding to Human KCNN4 aa 400 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in the following human tissue lysates: Skin Placenta
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab75956 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa).|
FunctionForms a voltage-independent potassium channel that is activated by intracellular calcium. Activation is followed by membrane hyperpolarization which promotes calcium influx. Required for maximal calcium influx and proliferation during the reactivation of naive T cells. The channel is blocked by clotrimazole and charybdotoxin but is insensitive to apamin.
Tissue specificityWidely expressed in non-excitable tissues.
Sequence similaritiesBelongs to the potassium channel KCNN family. KCa3.1/KCNN4 subfamily.
modificationsPhosphorylation at His-358 by NDKB activates the channel, and conversely it's dephosphorylation by PHPT1 inhibits the channel.
- Information by UniProt
- DHS2 antibody
- hIKCa1 antibody
- hKCa4 antibody
All lanes : Anti-KCNN4 antibody (ab75956) at 1 µg/ml
Lane 1 : Human skin tissue lysate - total protein (ab30166)
Lane 2 : Human placenta tissue lysate - total protein (ab29745)
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 47 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
The 50 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to KCNN4.
ab75956 has been referenced in 1 publication.
- Morales P et al. Contribution of the KCa3.1 channel-calmodulin interactions to the regulation of the KCa3.1 gating process. J Gen Physiol 142:37-60 (2013). WB . PubMed: 23797421