Key features and details
- Rabbit polyclonal to KCNN4 - C-terminal
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-KCNN4 antibody - C-terminal
See all KCNN4 primary antibodies
DescriptionRabbit polyclonal to KCNN4 - C-terminal
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide within Human KCNN4 (C terminal). The exact sequence is proprietary. Conjugated to a carrier protein.
Database link: O15554
- WB: Jurkat membrane and whole cell extracts.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229593 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 48 kDa.|
FunctionForms a voltage-independent potassium channel that is activated by intracellular calcium. Activation is followed by membrane hyperpolarization which promotes calcium influx. Required for maximal calcium influx and proliferation during the reactivation of naive T cells. The channel is blocked by clotrimazole and charybdotoxin but is insensitive to apamin.
Tissue specificityWidely expressed in non-excitable tissues.
Sequence similaritiesBelongs to the potassium channel KCNN family. KCa3.1/KCNN4 subfamily.
modificationsPhosphorylation at His-358 by NDKB activates the channel, and conversely it's dephosphorylation by PHPT1 inhibits the channel.
- Information by UniProt
- DHS2 antibody
- hIKCa1 antibody
- hKCa4 antibody
All lanes : Anti-KCNN4 antibody - C-terminal (ab229593) at 1/1000 dilution
Lane 1 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell extract
Lane 2 : Jurkat (Human T cell leukemia cell line from peripheral blood) membrane extract
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 48 kDa
10% SDS-PAGE gel.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229593 has not yet been referenced specifically in any publications.