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  1. Link

    kcnq1-antibody-n37a10-ab84819.pdf

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Neuroscience Neurotransmission Receptors / Channels Potassium Channels
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Anti-KCNQ1 antibody [N37A/10] (ab84819)

  • Datasheet
Submit a review Q&A (1)References (3)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNQ1 antibody [N37A/10] (ab84819)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNQ1 antibody [N37A/10] (ab84819)
  • Western blot - Anti-KCNQ1 antibody [N37A/10] (ab84819)
  • Flow Cytometry - Anti-KCNQ1 antibody [N37A/10] (ab84819)
  • Western blot - Anti-KCNQ1 antibody [N37A/10] (ab84819)

Key features and details

  • Mouse monoclonal [N37A/10] to KCNQ1
  • Suitable for: IHC-Fr, ICC/IF, IP, Flow Cyt, IHC-P, WB
  • Reacts with: Mouse, Rat, Human, Chinese hamster
  • Isotype: IgG1

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Overview

  • Product name

    Anti-KCNQ1 antibody [N37A/10]
    See all KCNQ1 primary antibodies
  • Description

    Mouse monoclonal [N37A/10] to KCNQ1
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-Fr, ICC/IF, IP, Flow Cyt, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Chinese hamster
  • Immunogen

    Fusion protein corresponding to Human KCNQ1 aa 1-150.
    Database link: P51787

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Lysate from transfected COS-1 cells transiently expressing KCNQ1.
  • General notes

    The clone number has been updated from S37A-10 to N37A/10, both clone numbers name the same antibody clone.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: PBS, 50% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    N37A/10
  • Isotype

    IgG1
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • Potassium Channels
    • Signal Transduction
    • Metabolism
    • Plasma Membrane
    • Channels
    • Cardiovascular
    • Heart
    • Cardiac arrhythmias
    • Metabolism
    • Types of disease
    • Cancer
    • Neuroscience
    • Processes

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab84819 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

IHC-P
Use a concentration of 0.1 - 1 µg/ml.
WB
Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 75 kDa.
Notes
IHC-Fr
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

IHC-P
Use a concentration of 0.1 - 1 µg/ml.
WB
Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 75 kDa.

Target

  • Function

    Potassium channel that plays an important role in a number of tissues, including heart, inner ear, stomach and colon (By similarity) (PubMed:10646604). Associates with KCNE beta subunits that modulates current kinetics (By similarity) (PubMed:9312006, PubMed:9108097, PubMed:8900283, PubMed:10646604, PubMed:11101505, PubMed:19687231). Induces a voltage-dependent by rapidly activating and slowly deactivating potassium-selective outward current (By similarity) (PubMed:9312006, PubMed:9108097, PubMed:8900283, PubMed:10646604, PubMed:11101505). Promotes also a delayed voltage activated potassium current showing outward rectification characteristic (By similarity). During beta-adrenergic receptor stimulation participates in cardiac repolarization by associating with KCNE1 to form the I(Ks) cardiac potassium current that increases the amplitude and slows down the activation kinetics of outward potassium current I(Ks) (By similarity) (PubMed:9312006, PubMed:9108097, PubMed:8900283, PubMed:10646604, PubMed:11101505). Muscarinic agonist oxotremorine-M strongly suppresses KCNQ1/KCNE1 current (PubMed:10713961). When associated with KCNE3, forms the potassium channel that is important for cyclic AMP-stimulated intestinal secretion of chloride ions (PubMed:10646604). This interaction with KCNE3 is reduced by 17beta-estradiol, resulting in the reduction of currents (By similarity). During conditions of increased substrate load, maintains the driving force for proximal tubular and intestinal sodium ions absorption, gastric acid secretion, and cAMP-induced jejunal chloride ions secretion (By similarity). Allows the provision of potassium ions to the luminal membrane of the secretory canaliculus in the resting state as well as during stimulated acid secretion (By similarity). When associated with KCNE2, forms a heterooligomer complex leading to currents with an apparently instantaneous activation, a rapid deactivation process and a linear current-voltage relationship and decreases the amplitude of the outward current (PubMed:11101505). When associated with KCNE4, inhibits voltage-gated potassium channel activity (PubMed:19687231). When associated with KCNE5, this complex only conducts current upon strong and continued depolarization (PubMed:12324418). Also forms a heterotetramer with KCNQ5; has a voltage-gated potassium channel activity (PubMed:24855057). Binds with phosphatidylinositol 4,5-bisphosphate (PubMed:25037568).
    Isoform 2: Non-functional alone but modulatory when coexpressed with the full-length isoform 1.
  • Tissue specificity

    Abundantly expressed in heart, pancreas, prostate, kidney, small intestine and peripheral blood leukocytes. Less abundant in placenta, lung, spleen, colon, thymus, testis and ovaries.
  • Involvement in disease

    Long QT syndrome 1
    Jervell and Lange-Nielsen syndrome 1
    Atrial fibrillation, familial, 3
    Short QT syndrome 2
    Diabetes mellitus, non-insulin-dependent
  • Sequence similarities

    Belongs to the potassium channel family. KQT (TC 1.A.1.15) subfamily. Kv7.1/KCNQ1 sub-subfamily.
  • Domain

    The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
    The coiled-coil domain mediates tetramerization.
    The segment S6 is involved in the inhibition of voltage-gated potassium channel activity by KCNE4.
    The C-terminal assembly domain promotes self-interactiona; allows functional channel.
  • Post-translational
    modifications

    Phosphorylation at Ser-27 by PKA; increases delayed rectifier potassium channel activity of the KCNQ1-KCNE1 complex through a macromolecular complex that includes PKA, PP1, and the targeting protein AKAP9.
    Ubiquitinated by NEDD4L; promotes internalization (PubMed:22024150). The ubiquitinylated form is internalized through a clathrin-mediated endocytosis by interacting with AP2M1 and is recycled back to the cell membrane via RAB4A and RAB11A (PubMed:23529131).
    Deubiquitinated by USP2; counteracts the NEDD4L-specific down-regulation of I(Ks) and restores the membrane localization.
  • Cellular localization

    Cell membrane. Cytoplasmic vesicle membrane. Early endosome. Membrane raft. Endoplasmic reticulum. Basolateral cell membrane. Colocalized with KCNE3 at the plasma membrane (PubMed:10646604). Upon 17beta-oestradiol treatment, colocalizes with RAB5A at early endosome (PubMed:23529131). Heterotetramer with KCNQ5 is highly retained at the endoplasmic reticulum and is localized outside of lipid raft microdomains (PubMed:24855057). During the early stages of epithelial cell polarization induced by the calcium switch it removed from plasma membrane to the endoplasmic reticulum where it retained and it is redistributed to the basolateral cell surface in a PI3K-dependent manner at a later stage (PubMed:21228319).
  • Target information above from: UniProt accession P51787 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 100761481 Chinese hamster
    • Entrez Gene: 3784 Human
    • Entrez Gene: 16535 Mouse
    • Entrez Gene: 84020 Rat
    • Omim: 607542 Human
    • SwissProt: P51787 Human
    • SwissProt: P97414 Mouse
    • SwissProt: Q9Z0N7 Rat
    • Unigene: 95162 Human
    • Unigene: 439769 Mouse
    • Unigene: 453036 Mouse
    • Unigene: 453037 Mouse
    • Unigene: 9779 Rat
    see all
  • Form

    There are 2 isoforms produced by alternative splicing. Isoform 2 also known as: TKvLQT1.
  • Alternative names

    • ATFB1 antibody
    • ATFB3 antibody
    • FLJ26167 antibody
    • IKs producing slow voltage-gated potassium channel subunit alpha antibody
    • IKs producing slow voltage-gated potassium channel subunit alpha KvLQT1 antibody
    • Jervell and Lange-Nielsen syndrome 1 antibody
    • JLNS1 antibody
    • KCNA8 antibody
    • KCNA9 antibody
    • KCNQ1 antibody
    • KCNQ1_HUMAN antibody
    • kidney and cardiac voltage dependend K+ channel antibody
    • KQT-like 1 antibody
    • Kv1.9 antibody
    • Kv7.1 antibody
    • KVLQT1 antibody
    • long (electrocardiographic) QT syndrome, Ward-Romano syndrome 1 antibody
    • LQT antibody
    • LQT1 antibody
    • Potassium channel, voltage-gated, shaker-relatd subfamily, member 9 antibody
    • Potassium voltage-gated channel subfamily KQT member 1 antibody
    • potassium voltage-gated channel, KQT-like subfamily, member 1 antibody
    • RWS antibody
    • slow delayed rectifier channel subunit antibody
    • SQT2 antibody
    • Voltage-gated potassium channel subunit Kv7.1 antibody
    • WRS antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNQ1 antibody [N37A/10] (ab84819)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNQ1 antibody [N37A/10] (ab84819)

    ab84819 staining KCNQ1 in human hippocampal tissue by IHC-P (Bouin's fixative fixed paraffin embedded). The sample was incubated with primary antibody at 100 and with a fluorophore conjugated anti mouse secondary at 1/50 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNQ1 antibody [N37A/10] (ab84819)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNQ1 antibody [N37A/10] (ab84819)

    Immunohistochemical analysis of Mouse Brain labelled for KCNQ1 using ab84819. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-KCNQ1 Monoclonal Antibody (ab84819) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT.

  • Western blot - Anti-KCNQ1 antibody [N37A/10] (ab84819)
    Western blot - Anti-KCNQ1 antibody [N37A/10] (ab84819)
    Anti-KCNQ1 antibody [N37A/10] (ab84819) at 1/1000 dilution + Human Cell lysates at 15 µg

    Secondary
    Sheep Anti-Mouse IgG: HRP for 1 hour at RT

    Predicted band size: 75 kDa

  • Flow Cytometry - Anti-KCNQ1 antibody [N37A/10] (ab84819)
    Flow Cytometry - Anti-KCNQ1 antibody [N37A/10] (ab84819)
    Overlay histogram showing HEK293 cells stained with ab84819 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab84819, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Western blot - Anti-KCNQ1 antibody [N37A/10] (ab84819)
    Western blot - Anti-KCNQ1 antibody [N37A/10] (ab84819)
    All lanes : Anti-KCNQ1 antibody [N37A/10] (ab84819) at 1 µg/ml

    Lane 1 : Molecular weight marker
    Lane 2 : Cell lysates prepared from CHO -T cells transfected with mink-KvLQT1

    Predicted band size: 75 kDa

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (3)

Publishing research using ab84819? Please let us know so that we can cite the reference in this datasheet.

ab84819 has been referenced in 3 publications.

  • Hu D  et al. Identification, clinical manifestation and structural mechanisms of mutations in AMPK associated cardiac glycogen storage disease. EBioMedicine 54:102723 (2020). PubMed: 32259713
  • Egashira T  et al. Disease characterization using LQTS-specific induced pluripotent stem cells. Cardiovasc Res 95:419-29 (2012). PubMed: 22739119
  • Iqbal J  et al. Potassium and ANO1/ TMEM16A chloride channel profiles distinguish atypical and typical smooth muscle cells from interstitial cells in the mouse renal pelvis. Br J Pharmacol 165:2389-408 (2012). PubMed: 22014103

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

Question

Dear Abcam,
I am currently using two of your products (ab84819 - anti KCNQ1 antibody and ab65795 - anti KCNE1 antibody) for immunocytochemistry. I have had a look on your website for the synthetic peptides derived from each of these proteins, however I cannot seem to locate them. I require these for (negative) absorption controls for my experiments. Would you be able to tell me if this is a product that you do? If not, perhaps direct me to another suitable product.
Kind regards,

Read More

Abcam community

Verified customer

Asked on Nov 22 2012

Answer

Thank you for contacting us.

The blocking peptide for the rabbit polyclonal anti-KCNE1 is now available, the catalog reference number is ab151760 and the link to the datasheet is https://www.abcam.com/ab151760.

Unfortunately, the peptide used to raise the clone [S37A-10] is not commercially available.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.



Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Read More

Abcam Scientific Support

Answered on Nov 22 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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