Anti-KCNQ2 antibody [N26A/23] (ab84812)
Key features and details
- Mouse monoclonal [N26A/23] to KCNQ2
- Suitable for: IHC-P, IHC-Fr, WB, Flow Cyt
- Reacts with: Mouse, Human, Chinese hamster
- Isotype: IgG1
Overview
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Product name
Anti-KCNQ2 antibody [N26A/23]
See all KCNQ2 primary antibodies -
Description
Mouse monoclonal [N26A/23] to KCNQ2 -
Host species
Mouse -
Tested Applications & Species
Application Species Flow Cyt HumanIHC-Fr MouseIHC-P HumanWB Chinese hamster -
Immunogen
Fusion protein, corresponding to amino acids 1-59 of Human KCNQ2 (accession number O43526)
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Positive control
- COS-1 cell lysate transiently expressing KCNQ2
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General notes
The clone number has been updated from S26A-23 to N26A/23, both clone numbers name the same antibody clone.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
N26A/23 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab84812 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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Flow Cyt |
Human
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IHC-Fr |
Mouse
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IHC-P |
Human
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WB |
Chinese hamster
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Application | Abreviews | Notes |
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IHC-P |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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WB |
Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 95 kDa.
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Flow Cyt |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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Notes |
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IHC-P
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
WB
Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 95 kDa. |
Flow Cyt
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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Target
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Function
Probably important in the regulation of neuronal excitability. Associates with KCNQ3 to form a potassium channel with essentially identical properties to the channel underlying the native M-current, a slowly activating and deactivating potassium conductance which plays a critical role in determining the subthreshold electrical excitability of neurons as well as the responsiveness to synaptic inputs. KCNQ2/KCNQ3 current is blocked by linopirdine and XE991, and activated by the anticonvulsant retigabine. Muscarinic agonist oxotremorine-M strongly suppress KCNQ2/KCNQ3 current in cells in which cloned KCNQ2/KCNQ3 channels were coexpressed with M1 muscarinic receptors. -
Tissue specificity
In adult and fetal brain. Highly expressed in areas containing neuronal cell bodies, low in spinal chord and corpus callosum. Isoform 2 is preferentially expressed in differentiated neurons. Isoform 6 is prominent in fetal brain, undifferentiated neuroblastoma cells and brain tumors. -
Involvement in disease
Defects in KCNQ2 are the cause of benign familial neonatal seizures type 1 (BFNS1) [MIM:121200]. A disorder characterized by clusters of seizures occurring in the first days of life. Most patients have spontaneous remission by 12 months of age and show normal psychomotor development. Some rare cases manifest an atypical severe phenotype associated with epileptic encephalopathy and psychomotor retardation. The disorder is distinguished from benign familial infantile seizures by an earlier age at onset. In some patients, neonatal convulsions are followed later in life by myokymia, a benign condition characterized by spontaneous involuntary contractions of skeletal muscles fiber groups that can be observed as vermiform movement of the overlying skin. Electromyography typically shows continuous motor unit activity with spontaneous oligo- and multiplet-discharges of high intraburst frequency (myokymic discharges). Some patients may have isolated myokymia.
Defects in KCNQ2 are the cause of epileptic encephalopathy early infantile type 7 (EIEE7) [MIM:613720]. EIEE7 is an autosomal dominant seizure disorder characterized by infantile onset of refractory seizures with resultant delayed neurologic development and persistent neurologic abnormalities. -
Sequence similarities
Belongs to the potassium channel family. KQT (TC 1.A.1.15) subfamily. Kv7.2/KCNQ2 sub-subfamily. -
Domain
The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position. -
Post-translational
modificationsIn Xenopus oocytes KCNQ2/KCNQ3 heteromeric current can be increased by intracellular cyclic AMP, an effect that depends on phosphorylation of Ser-52 in the N-terminus region. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3785 Human
- Entrez Gene: 16536 Mouse
- Omim: 602235 Human
- SwissProt: O43526 Human
- SwissProt: Q5VYU0 Human
- SwissProt: Q5VYU1 Human
- SwissProt: Q5VYU2 Human
- SwissProt: Q5VYU3 Human
see all -
Alternative names
- BFNC antibody
- BFNS1 antibody
- EBN 1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNQ2 antibody [N26A/23] (ab84812)ab84812 staining KCNQ2 in human hippocampal tissue by IHC-P (Bouin's fixed paraffin embedded).
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Overlay histogram showing SH-SH5Y cells stained with ab84812 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab84812, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
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All lanes : Anti-KCNQ2 antibody [N26A/23] (ab84812) at 10 µg/ml
Lane 1 : CHO cell lysate
Lane 2 : HEK cell lysate
Predicted band size: 95 kDa -
ab84812 staining KCNQ2 in Mouse brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab84812 has been referenced in 1 publication.
- Nieto-Gonzalez JL & Jensen K BDNF Depresses Excitability of Parvalbumin-Positive Interneurons through an M-Like Current in Rat Dentate Gyrus. PLoS One 8:e67318 (2013). PubMed: 23840662