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    kcnq2-antibody-n26a23-ab84812.pdf

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Neuroscience Neurotransmission Receptors / Channels Potassium Channels
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Anti-KCNQ2 antibody [N26A/23] (ab84812)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (1)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNQ2 antibody [N26A/23] (ab84812)
  • Flow Cytometry - Anti-KCNQ2 antibody [N26A/23] (ab84812)
  • Western blot - Anti-KCNQ2 antibody [N26A/23] (ab84812)
  • Immunohistochemistry (Frozen sections) - Anti-KCNQ2 antibody [N26A/23] (ab84812)

Key features and details

  • Mouse monoclonal [N26A/23] to KCNQ2
  • Suitable for: IHC-P, IHC-Fr, WB, Flow Cyt
  • Reacts with: Mouse, Human, Chinese hamster
  • Isotype: IgG1

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Overview

  • Product name

    Anti-KCNQ2 antibody [N26A/23]
    See all KCNQ2 primary antibodies
  • Description

    Mouse monoclonal [N26A/23] to KCNQ2
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-Fr
    Mouse
    IHC-P
    Human
    WB
    Chinese hamster
    See all applications and species data
  • Immunogen

    Fusion protein, corresponding to amino acids 1-59 of Human KCNQ2 (accession number O43526)

  • Positive control

    • COS-1 cell lysate transiently expressing KCNQ2
  • General notes

    The clone number has been updated from S26A-23 to N26A/23, both clone numbers name the same antibody clone.

     

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: PBS, 50% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    N26A/23
  • Isotype

    IgG1
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • Potassium Channels
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Other

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab84812 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
IHC-Fr
Mouse
IHC-P
Human
WB
Chinese hamster
Application Abreviews Notes
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
WB
Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 95 kDa.
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Notes
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
WB
Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 95 kDa.
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Probably important in the regulation of neuronal excitability. Associates with KCNQ3 to form a potassium channel with essentially identical properties to the channel underlying the native M-current, a slowly activating and deactivating potassium conductance which plays a critical role in determining the subthreshold electrical excitability of neurons as well as the responsiveness to synaptic inputs. KCNQ2/KCNQ3 current is blocked by linopirdine and XE991, and activated by the anticonvulsant retigabine. Muscarinic agonist oxotremorine-M strongly suppress KCNQ2/KCNQ3 current in cells in which cloned KCNQ2/KCNQ3 channels were coexpressed with M1 muscarinic receptors.
  • Tissue specificity

    In adult and fetal brain. Highly expressed in areas containing neuronal cell bodies, low in spinal chord and corpus callosum. Isoform 2 is preferentially expressed in differentiated neurons. Isoform 6 is prominent in fetal brain, undifferentiated neuroblastoma cells and brain tumors.
  • Involvement in disease

    Defects in KCNQ2 are the cause of benign familial neonatal seizures type 1 (BFNS1) [MIM:121200]. A disorder characterized by clusters of seizures occurring in the first days of life. Most patients have spontaneous remission by 12 months of age and show normal psychomotor development. Some rare cases manifest an atypical severe phenotype associated with epileptic encephalopathy and psychomotor retardation. The disorder is distinguished from benign familial infantile seizures by an earlier age at onset. In some patients, neonatal convulsions are followed later in life by myokymia, a benign condition characterized by spontaneous involuntary contractions of skeletal muscles fiber groups that can be observed as vermiform movement of the overlying skin. Electromyography typically shows continuous motor unit activity with spontaneous oligo- and multiplet-discharges of high intraburst frequency (myokymic discharges). Some patients may have isolated myokymia.
    Defects in KCNQ2 are the cause of epileptic encephalopathy early infantile type 7 (EIEE7) [MIM:613720]. EIEE7 is an autosomal dominant seizure disorder characterized by infantile onset of refractory seizures with resultant delayed neurologic development and persistent neurologic abnormalities.
  • Sequence similarities

    Belongs to the potassium channel family. KQT (TC 1.A.1.15) subfamily. Kv7.2/KCNQ2 sub-subfamily.
  • Domain

    The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
  • Post-translational
    modifications

    In Xenopus oocytes KCNQ2/KCNQ3 heteromeric current can be increased by intracellular cyclic AMP, an effect that depends on phosphorylation of Ser-52 in the N-terminus region.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession O43526 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3785 Human
    • Entrez Gene: 16536 Mouse
    • Omim: 602235 Human
    • SwissProt: O43526 Human
    • SwissProt: Q5VYU0 Human
    • SwissProt: Q5VYU1 Human
    • SwissProt: Q5VYU2 Human
    • SwissProt: Q5VYU3 Human
    • SwissProt: Q5VYU4 Human
    • SwissProt: Q5VYU5 Human
    • SwissProt: Q5VYU6 Human
    • SwissProt: Q9Z351 Mouse
    • Unigene: 161851 Human
    • Unigene: 652468 Human
    • Unigene: 40615 Mouse
    • Unigene: 440175 Mouse
    see all
  • Alternative names

    • BFNC antibody
    • BFNS1 antibody
    • EBN 1 antibody
    • EBN antibody
    • EBN1 antibody
    • EIEE7 antibody
    • ENB 1 antibody
    • ENB1 antibody
    • HNSPC antibody
    • KCNA 11 antibody
    • KCNA11 antibody
    • KCNQ 2 antibody
    • Kcnq2 antibody
    • KCNQ2_HUMAN antibody
    • KQT like 2 antibody
    • KQT-like 2 antibody
    • KV7.2 antibody
    • KVEBN 1 antibody
    • KVEBN1 antibody
    • KvLQT 2 antibody
    • KvLQT2 antibody
    • Neuroblastoma specific potassium channel alpha subunit KvLQT2 antibody
    • Neuroblastoma specific potassium channel protein antibody
    • Neuroblastoma specific potassium channel subunit alpha antibody
    • Neuroblastoma specific potassium channel subunit alpha KvLQT2 antibody
    • Neuroblastoma-specific potassium channel subunit alpha KvLQT2 antibody
    • OTTHUMP00000031681 antibody
    • OTTHUMP00000031682 antibody
    • OTTHUMP00000031684 antibody
    • OTTHUMP00000031685 antibody
    • OTTHUMP00000031686 antibody
    • OTTHUMP00000031687 antibody
    • OTTHUMP00000031689 antibody
    • Potassium voltage gated channel KQT like protein 2 antibody
    • Potassium voltage gated channel KQT like subfamily member 2 antibody
    • Potassium voltage gated channel subfamily KQT member 2 antibody
    • Potassium voltage-gated channel subfamily KQT member 2 antibody
    • Voltage gated potassium channel subunit Kv7.2 antibody
    • Voltage-gated potassium channel subunit Kv7.2 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNQ2 antibody [N26A/23] (ab84812)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNQ2 antibody [N26A/23] (ab84812)
    ab84812 staining KCNQ2 in human hippocampal tissue by IHC-P (Bouin's fixed paraffin embedded).
  • Flow Cytometry - Anti-KCNQ2 antibody [N26A/23] (ab84812)
    Flow Cytometry - Anti-KCNQ2 antibody [N26A/23] (ab84812)

    Overlay histogram showing SH-SH5Y cells stained with ab84812 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab84812, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Western blot - Anti-KCNQ2 antibody [N26A/23] (ab84812)
    Western blot - Anti-KCNQ2 antibody [N26A/23] (ab84812)
    All lanes : Anti-KCNQ2 antibody [N26A/23] (ab84812) at 10 µg/ml

    Lane 1 : CHO cell lysate
    Lane 2 : HEK cell lysate

    Predicted band size: 95 kDa

  • Immunohistochemistry (Frozen sections) - Anti-KCNQ2 antibody [N26A/23] (ab84812)
    Immunohistochemistry (Frozen sections) - Anti-KCNQ2 antibody [N26A/23] (ab84812)

    ab84812 staining KCNQ2 in Mouse brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections).

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (1)

Publishing research using ab84812? Please let us know so that we can cite the reference in this datasheet.

ab84812 has been referenced in 1 publication.

  • Nieto-Gonzalez JL & Jensen K BDNF Depresses Excitability of Parvalbumin-Positive Interneurons through an M-Like Current in Rat Dentate Gyrus. PLoS One 8:e67318 (2013). PubMed: 23840662

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

Question

Product code: 84812
Lot number: GR27740-1
Inquiry: Hi I ordered and received KCNQ2 antibody (AB84812) , but almost 40 ul antibody was leaked like a attached image. Could you change to a new one? Thanks.

Read More

Abcam community

Verified customer

Asked on Aug 21 2012

Answer

Thank you for your email.

I am sorry this product did not contain the amountas stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Read More

Abcam Scientific Support

Answered on Aug 21 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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