Overview

  • Product name

  • Description

    Rabbit polyclonal to KCNQ4
  • Host species

    Rabbit
  • Specificity

    This antibody reacts specifically with KCNQ4 protein.
  • Tested applications

    Suitable for: ICC/IF, IHC-Fr, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide derived from the N terminal domain of KCNQ4 protein

Properties

Applications

Our Abpromise guarantee covers the use of ab65797 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 23242999
IHC-Fr Use at an assay dependent concentration. PubMed: 21740972
WB 1/500 - 1/5000. Predicted molecular weight: 77 kDa.

Target

  • Function

    Probably important in the regulation of neuronal excitability. May underlie a potassium current involved in regulating the excitability of sensory cells of the cochlea. KCNQ4 channels are blocked by linopirdin, XE991 and bepridil, whereas clofilium is without significant effect. Muscarinic agonist oxotremorine-M strongly suppress KCNQ4 current in CHO cells in which cloned KCNQ4 channels were coexpressed with M1 muscarinnic receptors.
  • Tissue specificity

    Expressed in the outer, but not the inner, sensory hair cells of the cochlea. Slightly expressed in heart, brain and skeletal muscle.
  • Involvement in disease

    Defects in KCNQ4 are the cause of deafness autosomal dominant type 2A (DFNA2A) [MIM:600101]. DFNA2A is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
  • Sequence similarities

    Belongs to the potassium channel family. KQT (TC 1.A.1.15) subfamily. Kv7.4/KCNQ4 sub-subfamily.
  • Domain

    The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
    The A-domain tail carries the major determinants of channel assembly specificity. Its coiled-coil region is Four-stranded.
  • Cellular localization

    Basal cell membrane. Situated at the basal membrane of cochlear outer hair cells.
  • Information by UniProt
  • Database links

  • Alternative names

    • DFNA 2 antibody
    • DFNA2 antibody
    • KCNQ 4 antibody
    • Kcnq4 antibody
    • KCNQ4_HUMAN antibody
    • KQT like 4 antibody
    • KQT-like 4 antibody
    • KV7.4 antibody
    • Potassium channel KQT like 4 antibody
    • Potassium channel subunit alpha KvLQT4 antibody
    • Potassium voltage gated channel KQT like protein 4 antibody
    • Potassium voltage gated channel KQT like subfamily member 4 antibody
    • Potassium voltage gated channel subfamily KQT member 4 antibody
    • Potassium voltage-gated channel subfamily KQT member 4 antibody
    • Voltage gated potassium channel subunit Kv7.4 antibody
    • Voltage-gated potassium channel subunit Kv7.4 antibody
    see all

References

This product has been referenced in:

  • Wei X  et al. The expression and function of KCNQ potassium channels in human chorionic plate arteries from women with normal pregnancies and pre-eclampsia. PLoS One 13:e0192122 (2018). Read more (PubMed: 29579054) »
  • Morales-Cano D  et al. Kv7 channels critically determine coronary artery reactivity: left-right differences and down-regulation by hyperglycaemia. Cardiovasc Res 106:98-108 (2015). Read more (PubMed: 25616413) »
See all 7 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Abreviews
Application
Western blot
Sample
Monkey Tissue lysate - whole (colon)
Gel Running Conditions
Reduced Denaturing (8)
Loading amount
20 µg
Specification
colon
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1µg/mL · Temperature: RT°C

Abcam user community

Verified customer

Submitted May 30 2018

Answer

Is the Kv7.1 antibody also a rabbit polyclonal or was this raised in a different host species? Generally issues like this arise from an antibody (usually the secondary but sometimes the primary) reacting to the proteins within the blocking reagent. Have you had the chance to test this product with another secondary antibody or with a new blocking reagent such as 3-5% BSA in PBST or a casein buffer?

Read More

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