Anti-KCNQ4 antibody [N43/6] (ab84820)
Key features and details
- Mouse monoclonal [N43/6] to KCNQ4
- Suitable for: IHC-Fr, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-KCNQ4 antibody [N43/6] -
Description
Mouse monoclonal [N43/6] to KCNQ4 -
Host species
Mouse -
Tested Applications & Species
Application Species IHC-Fr MouseIHC-P HumanWB Rat -
Immunogen
Fusion protein: AEAPPRRLGL GPPPGDAPRA ELVALTAVQS EQGEAGGGGS PRRLGLLGSP LPPGAPLPGP GSGSGSACGQ RSSAAH, corresponding to amino acids 2-77 of Human KCNQ4 (accession number P56696).
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Positive control
- Lysate from transfected COS-1 cells transiently expressing KCNQ4.
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General notes
The clone number has been updated from S43-6 to N43/6, both clone numbers name the same antibody clone.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol, PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
N43/6 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab84820 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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IHC-Fr |
Mouse
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IHC-P |
Human
|
WB |
Rat
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All applications |
Chimpanzee
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Application | Abreviews | Notes |
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IHC-Fr |
1/1000.
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IHC-P |
Use at an assay dependent concentration.
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|
WB |
1/1000. Predicted molecular weight: 77 kDa.
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Notes |
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IHC-Fr
1/1000. |
IHC-P
Use at an assay dependent concentration. |
WB
1/1000. Predicted molecular weight: 77 kDa. |
Target
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Function
Probably important in the regulation of neuronal excitability. May underlie a potassium current involved in regulating the excitability of sensory cells of the cochlea. KCNQ4 channels are blocked by linopirdin, XE991 and bepridil, whereas clofilium is without significant effect. Muscarinic agonist oxotremorine-M strongly suppress KCNQ4 current in CHO cells in which cloned KCNQ4 channels were coexpressed with M1 muscarinnic receptors. -
Tissue specificity
Expressed in the outer, but not the inner, sensory hair cells of the cochlea. Slightly expressed in heart, brain and skeletal muscle. -
Involvement in disease
Defects in KCNQ4 are the cause of deafness autosomal dominant type 2A (DFNA2A) [MIM:600101]. DFNA2A is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. -
Sequence similarities
Belongs to the potassium channel family. KQT (TC 1.A.1.15) subfamily. Kv7.4/KCNQ4 sub-subfamily. -
Domain
The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
The A-domain tail carries the major determinants of channel assembly specificity. Its coiled-coil region is Four-stranded. -
Cellular localization
Basal cell membrane. Situated at the basal membrane of cochlear outer hair cells. - Information by UniProt
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Database links
- Entrez Gene: 456800 Chimpanzee
- Entrez Gene: 9132 Human
- Entrez Gene: 60613 Mouse
- Entrez Gene: 298496 Rat
- Omim: 603537 Human
- SwissProt: P56696 Human
- SwissProt: Q9JK97 Mouse
- SwissProt: Q9JK96 Rat
see all -
Alternative names
- DFNA 2 antibody
- DFNA2 antibody
- KCNQ 4 antibody
see all
Images
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ab84820 staining KCNQ4 in Mouse brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections).
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All lanes : Anti-KCNQ4 antibody [N43/6] (ab84820)
Lane 1 : Molecular weight marker
Lane 2 : Mixed cell lysates prepared from rat tissues Brain, Heart, Kidney, Liver, Lung, Pancreas, Skeletal Muscle, Spleen, Testes, Thymus, and H9C2 cells at 10 µg
Predicted band size: 77 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNQ4 antibody [N43/6] (ab84820)ab84820 staining KCNQ4 in human hippocampal tissue by IHC-P (Bouin's fixed paraffin embedded).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (2)
ab84820 has been referenced in 2 publications.
- Brueggemann LI et al. Differential protein kinase C-dependent modulation of Kv7.4 and Kv7.5 subunits of vascular Kv7 channels. J Biol Chem 289:2099-111 (2014). WB, ICC/IF . PubMed: 24297175
- Udagawa T et al. Inwardly rectifying potassium channel Kir4.1 is localized at the calyx endings of vestibular afferents. Neuroscience 215:209-16 (2012). PubMed: 22546335