Overview

  • Product name
    Anti-KCNQ4 antibody [N43/6]
    See all KCNQ4 primary antibodies
  • Description
    Mouse monoclonal [N43/6] to KCNQ4
  • Host species
    Mouse
  • Tested applications
    Suitable for: IHC-Fr, IHC-P, WB, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chimpanzee
  • Immunogen

    Fusion protein: AEAPPRRLGL GPPPGDAPRA ELVALTAVQS EQGEAGGGGS PRRLGLLGSP LPPGAPLPGP GSGSGSACGQ RSSAAH, corresponding to amino acids 2-77 of Human KCNQ4 (accession number P56696).

  • Positive control
    • Lysate from transfected COS-1 cells transiently expressing KCNQ4.
  • General notes

    The clone number has been updated from S43-6 to N43/6, both clone numbers name the same antibody clone.

     

Properties

Applications

Our Abpromise guarantee covers the use of ab84820 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/1000.
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 - 10 mg/ml. Predicted molecular weight: 77 kDa.
ICC/IF Use a concentration of 1 - 10 µg/ml.
IP Use at an assay dependent concentration.

Target

  • Function
    Probably important in the regulation of neuronal excitability. May underlie a potassium current involved in regulating the excitability of sensory cells of the cochlea. KCNQ4 channels are blocked by linopirdin, XE991 and bepridil, whereas clofilium is without significant effect. Muscarinic agonist oxotremorine-M strongly suppress KCNQ4 current in CHO cells in which cloned KCNQ4 channels were coexpressed with M1 muscarinnic receptors.
  • Tissue specificity
    Expressed in the outer, but not the inner, sensory hair cells of the cochlea. Slightly expressed in heart, brain and skeletal muscle.
  • Involvement in disease
    Defects in KCNQ4 are the cause of deafness autosomal dominant type 2A (DFNA2A) [MIM:600101]. DFNA2A is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
  • Sequence similarities
    Belongs to the potassium channel family. KQT (TC 1.A.1.15) subfamily. Kv7.4/KCNQ4 sub-subfamily.
  • Domain
    The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
    The A-domain tail carries the major determinants of channel assembly specificity. Its coiled-coil region is Four-stranded.
  • Cellular localization
    Basal cell membrane. Situated at the basal membrane of cochlear outer hair cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • DFNA 2 antibody
    • DFNA2 antibody
    • KCNQ 4 antibody
    • Kcnq4 antibody
    • KCNQ4_HUMAN antibody
    • KQT like 4 antibody
    • KQT-like 4 antibody
    • KV7.4 antibody
    • Potassium channel KQT like 4 antibody
    • Potassium channel subunit alpha KvLQT4 antibody
    • Potassium voltage gated channel KQT like protein 4 antibody
    • Potassium voltage gated channel KQT like subfamily member 4 antibody
    • Potassium voltage gated channel subfamily KQT member 4 antibody
    • Potassium voltage-gated channel subfamily KQT member 4 antibody
    • Voltage gated potassium channel subunit Kv7.4 antibody
    • Voltage-gated potassium channel subunit Kv7.4 antibody
    see all

Images

  • ab84820 staining KCNQ4 in KCNQ4 transfected NIH3T3 cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA, permeabilized with 0.5% Triton X-100 in PBS and blocked with 4% Milk in PBS for 60 minutes at 21°C. Samples were incubated with primary antibody (1/1000 in PBS) for 1 hour at 21°C. An Alexa Fluor®594-conjugated Goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • ab84820 staining KCNQ4 in Mouse brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections).

  • All lanes : Anti-KCNQ4 antibody [N43/6] (ab84820)

    Lane 1 : Molecular weight marker
    Lane 2 : Mixed cell lysates prepared from rat tissues Brain, Heart, Kidney, Liver, Lung, Pancreas, Skeletal Muscle, Spleen, Testes, Thymus, and H9C2 cells at 10 µg

    Predicted band size: 77 kDa

  • ab84820 staining KCNQ4 in human hippocampal tissue by IHC-P (Bouin's fixed paraffin embedded).

References

This product has been referenced in:
  • Brueggemann LI  et al. Differential protein kinase C-dependent modulation of Kv7.4 and Kv7.5 subunits of vascular Kv7 channels. J Biol Chem 289:2099-111 (2014). WB, ICC/IF . Read more (PubMed: 24297175) »
  • Udagawa T  et al. Inwardly rectifying potassium channel Kir4.1 is localized at the calyx endings of vestibular afferents. Neuroscience 215:209-16 (2012). Read more (PubMed: 22546335) »
See all 2 Publications for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

Filter by Ratings

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: RT°C
Sample
Mouse Cell (NIH3T3 TRANSFECTED KCNQ4 CELL)
Specification
NIH3T3 TRANSFECTED KCNQ4 CELL
Permeabilization
Yes - triton X100 0.5%
Fixative
Paraformaldehyde

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Submitted Oct 31 2014

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