Key features and details
- Mouse monoclonal [N3/26] to KCNT1/SLACK
- Suitable for: Flow Cyt, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Product nameAnti-KCNT1/SLACK antibody [N3/26]
See all KCNT1/SLACK primary antibodies
DescriptionMouse monoclonal [N3/26] to KCNT1/SLACK
Tested applicationsSuitable for: Flow Cyt, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
- Flow Cyt: SH-SY5Y cells. WB: Rat brain lysate.
The clone number has been updated from S3-26 to N3/26, both clone numbers name the same antibody clone.
This product was previously labelled as KCNT1
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab94578 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|WB||1/1000. Predicted molecular weight: 137 kDa.|
FunctionOutwardly rectifying potassium channel subunit that may co-assemble with other Slo-type channel subunits. Activated by high intracellular sodium or chloride levels. Activated upon stimulation of G-protein coupled receptors, such as CHRM1 and GRIA1. May be regulated by calcium in the absence of sodium ions (in vitro).
Tissue specificityHighest expression in liver, brain and spinal cord. Lowest expression in skeletal muscle.
Sequence similaritiesBelongs to the potassium channel family. Calcium-activated (TC 1.A.1.3) subfamily. KCa4.1/KCNT1 sub-subfamily.
Contains 1 RCK N-terminal domain.
modificationsPhosphorylated by protein kinase C. Phosphorylation of the C-terminal domain increases channel activity.
Cellular localizationCell membrane.
- Information by UniProt
- bA100C15.2 antibody
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Anti-KCNT1/SLACK antibody [N3/26] (ab94578) at 1/1000 dilution + Rat brain lysate at 15 µg
HRP-conjugated sheet anti-mouse IgG
Predicted band size: 137 kDa
Membrane was blocked with 1.5% BSA for 30 minutes at room temperature. Primari antibody was incubated for 2 hours at room temperature.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of mouse backskin staining KCNT1/SLACK with ab94578 at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.
Overlay histogram showing SH-SY5Y cells stained with ab94578 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab94578, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab94578 has not yet been referenced specifically in any publications.