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    kcnu1-antibody-n216-c-terminal-ab94586.pdf

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Neuroscience Neurotransmission Receptors / Channels Potassium Channels
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Anti-KCNU1 antibody [N2/16] - C-terminal (ab94586)

  • Datasheet
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Western blot - Anti-KCNU1 antibody [N2/16] - C-terminal (ab94586)

    Key features and details

    • Mouse monoclonal [N2/16] to KCNU1 - C-terminal
    • Suitable for: WB
    • Reacts with: Rat
    • Isotype: IgG2b

    You may also be interested in

    Secondary
    Product image
    Goat Anti-Mouse IgG H&L (HRP) (ab205719)

    View more associated products

    Overview

    • Product name

      Anti-KCNU1 antibody [N2/16] - C-terminal
    • Description

      Mouse monoclonal [N2/16] to KCNU1 - C-terminal
    • Host species

      Mouse
    • Tested Applications & Species

      Application Species
      WB
      Rat
      See all applications and species data
    • Immunogen

      Fusion protein corresponding to Mouse KCNU1 aa 1052-1121 (C terminal). AAB99742
      Sequence:

      DSSPSIQAQNNSTNATTPLAQGSNFFDSHHADESHDLYPVDDTGERWSQH HHSRVYPLDTLDASDIVQEK


      Database link: O54982
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • Positive control

      • WB: Rat brain membrane lysate.
    • General notes

      The clone number has been updated from S2-16 to N2/16, both clone numbers name the same antibody clone.

       

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
    • Storage buffer

      Preservative: 0.09% Sodium azide
      Constituents: 50% Glycerol (glycerin, glycerine), PBS
    • Concentration information loading...
    • Purity

      Protein G purified
    • Clonality

      Monoclonal
    • Clone number

      N2/16
    • Isotype

      IgG2b
    • Research areas

      • Neuroscience
      • Neurotransmission
      • Receptors / Channels
      • Potassium Channels
      • Developmental Biology
      • Reproduction
      • Fertilisation
      • Developmental Biology
      • Reproduction
      • Germ cell markers

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab94586 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Rat
    All applications
    Mouse
    Application Abreviews Notes
    WB
    Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 130 kDa.
    Notes
    WB
    Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 130 kDa.

    Target

    • Function

      Testis-specific potassium channel activated by both intracellular pH and membrane voltage that mediates export of K(+). May represent the primary spermatozoan K(+) current. In contrast to KCNMA1/SLO1, it is not activated by Ca(2+) or Mg(2+). Critical for fertility. May play an important role in sperm osmoregulation required for the acquisition of normal morphology and motility when faced with osmotic challenges, such as those experienced after mixing with seminal fluid and entry into the vagina.
    • Tissue specificity

      Testis-specific.
    • Sequence similarities

      Belongs to the potassium channel family. Calcium-activated (TC 1.A.1.3) subfamily. KCa5.1/KCNU1 sub-subfamily.
      Contains 1 RCK N-terminal domain.
    • Domain

      The S4 segment, which is characterized by a series of positively charged amino acids at every third position, is part of the voltage-sensor.
      The pore-forming domain (also referred as P region) is imbedded into the membrane, and forms the selectivity filter of the pore. It contains the signature sequence of potassium channels that displays selectivity to potassium.
      The RCK N-terminal domain mediates the homotetramerization, thereby promoting the assembly of monomers into functional potassium channel.
      The C-terminal cytosolic region confers the pH-dependence.
    • Cellular localization

      Cell membrane.
    • Target information above from: UniProt accession A8MYU2 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 16532 Mouse
      • Entrez Gene: 680912 Rat
      • SwissProt: O54982 Mouse
      • Unigene: 289679 Mouse
      • Alternative names

        • subfamily M subunit alpha-3 antibody
        • Calcium activated potassium channel subfamily M subunit alpha 3 antibody
        • Calcium activated potassium channel subunit alpha 3 antibody
        • Calcium-activated potassium channel antibody
        • Calcium-activated potassium channel subunit alpha-3 antibody
        • KCa5 antibody
        • KCa5.1 antibody
        • Kcnma3 antibody
        • KCNMC1 antibody
        • KCNU 1 antibody
        • KCNU1 antibody
        • KCNU1_HUMAN antibody
        • Potassium channel subfamily U member 1 antibody
        • Slo3 antibody
        • Slowpoke homolog 3 antibody
        see all

      Images

      • Western blot - Anti-KCNU1 antibody [N2/16] - C-terminal (ab94586)
        Western blot - Anti-KCNU1 antibody [N2/16] - C-terminal (ab94586)
        Anti-KCNU1 antibody [N2/16] - C-terminal (ab94586) at 1/1000 dilution + Rat brain membrane lysate at 15 µg

        Secondary
        Sheep Anti-Mouse IgG: HRP

        Predicted band size: 130 kDa



        Block: 1.5% BSA for 30 minutes at RT.

        Primary antibody incubated for 2 hours at RT.

        Secondary antibody incubated for 1 hour at RT.

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab94586? Please let us know so that we can cite the reference in this datasheet.

      ab94586 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Flow Cytometry abreview for Anti-KCNU1 antibody [N2/16] - C-terminal

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Flow Cytometry
      Sample
      Mouse Cell (spermatogenic)
      Permeabilization
      Yes - Tween 0.5%
      Gating Strategy
      spermatogenic cells
      Specification
      spermatogenic
      Preparation
      Cell harvesting/tissue preparation method: Enzymatic dissociation of testicular tissue
      Sample buffer: DMEM/Collagenase I
      Fixation
      Paraformaldehyde
      Read More

      Lis Puga

      Verified customer

      Submitted Mar 06 2020

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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