Overview

  • Product name
  • Description
    Rabbit polyclonal to KCTD12
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide within Human KCTD12 aa 248-278 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q96CX2

  • Positive control
    • T47D cells and lysates; Human brain tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab176201 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 36 kDa.

Target

  • Function
    Auxiliary subunit of GABA-B receptors that determine the pharmacology and kinetics of the receptor response. Increases agonist potency and markedly alter the G-protein signaling of the receptors by accelerating onset and promoting desensitization.
  • Tissue specificity
    Present in a variety of fetal organs, with highest expression levels in the cochlea and brain and, in stark contrast, is detected only at extremely low levels in adult organs, such as brain and lung.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Cell junction > synapse > presynaptic cell membrane. Cell junction > synapse > postsynaptic cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • C13orf2 antibody
    • BTB/POZ domain-containing protein KCTD12 antibody
    • KCD12_HUMAN antibody
    • KCTD12 antibody
    • KIAA1778 antibody
    • PFET1 antibody
    • Pfetin antibody
    • potassium channel tetramerisation domain containing 12 USA antibody
    • Predominantly fetal expressed T1 domain antibody
    see all

Images

  • Anti-KCTD12 antibody (ab176201) at 1/1000 dilution + T47D cell line lysates at 35 µg

    Predicted band size: 36 kDa

  • Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human brain tissue labeling KCTD12 with ab176201 at 1/50 diution, followed by peroxidase-conjugation to the secondary antibody, followed by DAB staining.

  • Flow cytometric analysis of T47D cells labeling KCTD12 with ab176201 at 1/10 dilution (right histogram), compared to a negative control cell (left histogram).FITC-conjugated Goat-anti-Rabbit secondary antibodies were used for the analysis.

References

ab176201 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab176201.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up