Key features and details
- Rabbit polyclonal to KCTD16
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-KCTD16 antibody
DescriptionRabbit polyclonal to KCTD16
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
- IHC-P: Human cerebral cortex tissue, WB: Transfected HEK-293T whole cell lysate, ICC: U-2 OS whole cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab185104 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100
|WB||Use a concentration of 0.04 - 0.4 mg/ml. Predicted molecular weight: 49 kDa.|
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionAuxiliary subunit of GABA-B receptors that determine the pharmacology and kinetics of the receptor response. Increases agonist potency and markedly alter the G-protein signaling of the receptors by accelerating onset and promoting desensitization.
Sequence similaritiesContains 1 BTB (POZ) domain.
Cellular localizationCell junction > synapse > presynaptic cell membrane. Cell junction > synapse > postsynaptic cell membrane.
- Information by UniProt
- BTB/POZ domain containing protein KCTD16 antibody
- BTB/POZ domain-containing protein KCTD16 antibody
- DKFZp781A1155 antibody
Immunohistochemical analysis of paraffin-embedded human cerebral cortex tissue labeling KCTD16 in the cytoplasm of neuronal cells with ab185104 at a 1/50 dilution.
All lanes : Anti-KCTD16 antibody (ab185104) at 0.4 µg/ml
Lane 1 : Control (vector only transfected) - HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : KCTD16 over-expression - HEK-293T whole cell lysate, (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa))
Predicted band size: 49 kDa
Immunocytochemistry analysis of U-2 OS (human bone osteosarcoma epithelial cell line) whole cells labeling KCTD16 in the nuclear speckles with ab185104 at 2 µg/ml.
ab185104 has not yet been referenced specifically in any publications.