Product nameAnti-KCTD20 antibody
DescriptionRabbit polyclonal to KCTD20
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
- Human anal skin tissue; RT-4 and U-251 MG cell lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab122094 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
|IHC-P||1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/250 - 1/500.|
Sequence similaritiesContains 1 BTB (POZ) domain.
- Information by UniProt
- BTB/POZ domain-containing protein KCTD20 antibody
- C6orf69 antibody
- dJ108K11.3 antibody
Immunofluorescent staining of Human cell line U-251MG shows positivity in endoplasmic reticulum. Recommended concentration of ab122094 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
ab122094, at 1/35 dilution, staining KCTD20 in paraffin-embedded Human anal skin tissue by Immunohistochemistry.
All lanes : Anti-KCTD20 antibody (ab122094) at 1/250 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human Plasma lysate
Lane 4 : Human Liver lysate
Lane 5 : Human Tonsil lysate
ab122094 has not yet been referenced specifically in any publications.